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pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Plasmid

#172514

Purpose

Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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OMM-long-CFAST10

Plasmid

#233598

Purpose

Expression of CFAST10 on the OMM membrane after a long linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-HA-mCherry-pA

Plasmid

#233030

Purpose

To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d-mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Macro-Linker-eGFP

Plasmid

#176067

Purpose

EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Macro

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-FHA-Linker-eGFP

Plasmid

#176065

Purpose

EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

FHA

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Beta-Globin-PTC39

Plasmid

#130701

Purpose

Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

Beta Globin (HBB Human)

Expression

Mammalian

Mutation

PTC39

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-WWE-Linker-eGFP

Plasmid

#176072

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

WWE

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-G protein Beta2 in pcDNA3.1

Plasmid

#42182

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Beta 2 (GNB2 Human)

Tags

Venus(156-239)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pLV-Hygro-EF1A-PBZC-Linker-eGFP

Plasmid

#176070

Purpose

EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PBZ-C

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-PBM-Linker-eGFP

Plasmid

#176069

Purpose

EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PBM

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-beta1(617-949)-GFP

Plasmid

#100732

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP1B1 (AP1B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF16_P2A_Hygro_Barcode

Plasmid

#120502

Purpose

Barcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF16 (KLF16 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRMPVneo.Yap.3093

Plasmid

#59915

Purpose

Tet on shRNA for conditional Yap1 knockdown

Depositor

Tyler Jacks

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

Yap1 (Yap1 Mouse)

Use

Retroviral

Available Since

Sept. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDule2-3-nitroTyrosine (5B)

Plasmid

#85499

Purpose

Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

Insert

3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Expression

Bacterial

Mutation

Y32H H70C D158S I159A L162R

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

Plasmid

#176542

Purpose

Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Inserts

MBP tag
TnpB

Tags

3' extension derived from endogenous locus a…

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-Hs4EHP_AE

Plasmid

#148588

Purpose

Mammalian Expression of Hs4EHP

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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TfR-moxGFP-DHFR

Plasmid

#173012

Purpose

For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

TfR-moxGFP-DHFR

Tags

DHFR and moxGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-TFEC

Plasmid

#178447

Purpose

doxycycline-inducible expression of Human TFEC in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

TFEC (TFEC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHS1928

Plasmid

#205967

Purpose

JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH all proteins, with His-tagged Cas8

Tags

14xHis on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1950

Plasmid

#205983

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Promoter

T7 (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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