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pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 stabilized mutant Beta Catenin-GFP
Plasmid#29684DepositorInsertbeta catenin (ctnnb1.L Frog)
UseTagsGFPExpressionMammalianMutationS33A, S37A, T41APromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
aP2-luciferase
Plasmid#8858DepositorInsertaP2 enhancer / promoter (Fabp4 Mouse)
UseLuciferaseTagsluciferase reporterExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcmvtag2Bmciap2 mut
Plasmid#11464DepositorInsertmciap2mut (Birc3 Mouse)
UseTagsflagExpressionMammalianMutationH570APromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS
Plasmid#199346PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLP710
Plasmid#203740PurposeIntegration of OsTIR1-3xMyc at NEUT5L locusDepositorInsertOsTIR1
UseCandida albicans integrating vectorTags3xMycExpressionMutationPromoterACT1 (C. albicans)Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-Rac1(Q61L)
Plasmid#22783DepositorInsertRac1 (Q61L) (RAC1 A. victoria, Human)
UseTagsCyPetExpressionMammalianMutationRac1 Q61L.PromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET22b-His6-UBCH7
Plasmid#212711PurposeBacterial expression of His6-UBCH7DepositorInsertUBCH7
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ii-OVA.II
Plasmid#64597PurposeExpression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentationDepositorInsertOvalbumin
UseTagsInvariant chainExpressionMammalianMutationOnly amino acids 315-353 (class II epitopes)PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo*
Plasmid#140159PurposeExpresses a regulatable secretory cargo (without an ER export signal) for mammalian cellsDepositorInsertpiGH-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP924 pHAGE2 CMV GFP
Plasmid#199392PurposeLentiviral transfer plasmid for constitutive expression of a GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP
Plasmid#199356PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Smad1 EVE GM
Plasmid#27997DepositorInsertHuman Smad1 EVE Gsk3 mutant (SMAD1 Human)
UseTagsFlagExpressionMammalianMutationS463E S465E S210A T202A S191A S198APromoterAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-26
Plasmid#160218PurposeAtpU6-26 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-26 promoter
UseGolden gate level 0 piece to express grnasTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAIP
Plasmid#74171PurposeLentiviral vector for expression of heterologous genesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-TTF-1-wt
Plasmid#74990PurposeRetroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNADepositorInsertTTF-1 (TTF1 Human)
UseRetroviralTagsExpressionMammalianMutationwild-type, full-length, without 3' UTRPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcmvtag2Bmciap2
Plasmid#11463DepositorInsertmciap2 (Birc3 Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-Rac1(T17N)
Plasmid#22784DepositorInsertRac1 (T17N) (RAC1 A. victoria, Human)
UseTagsCyPetExpressionMammalianMutationRac1 T17N.PromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21(+)-His6-Ubc13
Plasmid#212715PurposeBacterial expression of His6-Ubc13DepositorInsertUbc13
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pADH143
Plasmid#90989PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH140
Plasmid#90988PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.DepositorInsertsC. maltosa LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCaUra3-CUbo-AID*-GFP
Plasmid#212775PurposeC-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Ypet-PBD
Plasmid#24533DepositorInsertPAK binding domain (PAK1 Human)
UseRetroviralTagsYpetExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBlueRIP.TfrOVA
Plasmid#69596Purposecontains membrane bound form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-mOVA mice)DepositorInsertOVA (OVAL Chicken)
UseTagsTransmembrane domain of Transferrin Receptor (TfR…ExpressionMammalianMutationPromoterrat insulin promoter (RIP)Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-CyPet-Rac1
Plasmid#24534DepositorInsertRac1 (RAC1 Human)
UseRetroviralTagsCyPetExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS
Plasmid#199347PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-3CExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Muc2D1D2D3
Plasmid#155215PurposeExpresses human MUC2 residues 21-1259DepositorInsertMuc2 D1D2D3 (MUC2 Human)
UseTags6xHistadineExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-EEA-g1-PGK-Puro
Plasmid#102908PurposePiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneUseTagsGal4 Activation domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LZRS-GATA3
Plasmid#34836DepositorInsertGATA3 (Gata3 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutationPromoterLTRAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP
Plasmid#199357PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pADH139
Plasmid#90987PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.DepositorInsertgRNA conserved, C, mal LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW102
Plasmid#115945PurposeORF insert for replicon MoCloDepositorInsertLvl0 DDd-L7Ae
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
XE117 CAMKII-CS2+
Plasmid#16737DepositorInsertCAMKII (Camk2a Rat)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2 GFP-GSK3-MAPK-E3mut
Plasmid#29691DepositorInsertGFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK
UseTagsFlag and GFPExpressionMammalianMutationrecognition sites only, and E3 ligase site mutatedPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPotef-LN22*-3xGNLS-cbx
Plasmid#86780PurposeExpresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.DepositorInsertLN*
UseUstilago maydis expressionTagsGfpExpressionBacterialMutationPromoterPotefAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9GFP-shP53
Plasmid#102906PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.DepositorInsertdCas9GFP-shP53
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
SEPT5 Mut(S161A/S327A)
Plasmid#27275DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsMyc (MASMQKLISEEDL)ExpressionMammalianMutationS161A and S327APromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-flag-UTR
Plasmid#183234PurposeMammalian expression empty backboneDepositorTypeEmpty backboneUseTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Muc2D3
Plasmid#155216PurposeExpresses human Muc2 residues 858-1259DepositorInsertMuc2 D3 (MUC2 Human)
UseTags6xHistadineExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
SEPT5 Mut(S327A)
Plasmid#27274DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsMyc (MASMQKLISEEDL)ExpressionMammalianMutationS327APromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
XE93 mFZ3-CS2+
Plasmid#16804DepositorInsertmFZ3-CS2+ (Fzd3 Mouse)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTW324
Plasmid#115955PurposeDestination VectorDepositorInsertLvl1 P1 End
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW064
Plasmid#115951PurposeDestination VectorDepositorInsertLvl0 ORF Dest
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW063
Plasmid#115950PurposeDestination VectorDepositorInsertLvl0 SGP (+/- Apt) Dest
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
XE107 XPKC alpha-GFP-CS2+
Plasmid#16743DepositorInsertPKC-alpha (prkca Frog)
UseXenopus expressionTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only
