We narrowed to 12,317 results for: 112

1. pOP3GT

   Plasmid

   #112607

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GST fusions

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   Avi - 8His-GST

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOP5BT

   Plasmid

   #112609

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8His-GB1 and Avi

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSopBWT

   Plasmid

   #112512

   Purpose

   PsopB driven sopB and sigE

   Depositor

   Olivia Steele-Mortimer

   Article

   Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

   Insert

   sopB/sigE operon
   

   Expression

   Bacterial

   Promoter

   PsopB

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMV-gSWEET4

   Plasmid

   #112796

   Purpose

   Universal BeYDV-derived vector with gRNAs targeting the VviSWEET4 gene

   Depositor

   Humberto Prieto

   Article

   Prieto et al In: Cantu D., Walker M. (eds) The Grape

   Insert

   gRNA pair targeting VviSWEET4
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAT11

   Plasmid

   #112592

   Purpose

   Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His-MBP

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. rat APOBEC1 E63A

   Plasmid

   #112861

   Purpose

   plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Expression

   Mammalian

   Mutation

   changed Glutamic acid 63 to Alanine

   Promoter

   CMV promoter

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1673

   Plasmid

   #112047

   Purpose

   CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   SCT1
   

   Tags

   V5-TEV-EGFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

   Plasmid

   #112289

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   Human YAP1-2gamma lacking the last 5 Amino acids …

   Promoter

   EF-1 alpha

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mScarlet-I-LSS-SGFP2

   Plasmid

   #112945

   Purpose

   Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET

   Depositor

   Dorus Gadella

   Insert

   LSS-GFP2 mScarlet-I Fusion
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOP1

    Plasmid

    #112595

    Purpose

    Used for expression of protein in E. coli as non-fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJUNB-donor

    Plasmid

    #112313

    Purpose

    CRISPR donor plasmid to tag human transcription factor JUNB with GFP

    Depositor

    Kevin White

    Insert

    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    

    Use

    CRISPR

    Mutation

    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid

    #112288

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    3 Tyrosines (341, 357 and 394 corresponding to ty…

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-MBP-creM

    Plasmid

    #112238

    Purpose

    pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM

    Depositor

    Emily Balskus

    Article

    Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

    Insert

    CreM
    

    Tags

    MBP

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA6 MCV LTco S147A

    Plasmid

    #112192

    Purpose

    Expression of MCPyV LT mutant (Ser 147 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LUC56 (Luc/UGA/SP)

    Plasmid

    #112147

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258mutated to selenocysteine (U)

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. RhoA sensor (LSS-SGFP2-Scarlet-I)

    Plasmid

    #112947

    Purpose

    RhoA FRET biosensor

    Depositor

    Dorus Gadella

    Insert

    RhoA sensor
    

    Tags

    LSS-SGFP2 and mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEY1.1.0-gDNA

    Plasmid

    #112393

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1

    Depositor

    Kevin White

    Insert

    HEY1 (HEY1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHEY1-donor

    Plasmid

    #112297

    Purpose

    CRISPR donor plasmid to tag human transcription factor HEY1 with GFP

    Depositor

    Kevin White

    Insert

    HEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
    

    Use

    CRISPR

    Mutation

    hg38:8:79765311:G>A

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid

    #112489

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K377R

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSNAI2-donor

    Plasmid

    #112378

    Purpose

    CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP

    Depositor

    Kevin White

    Insert

    SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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