We narrowed to 2,497 results for: ERK

1. pcDNA3.1(+)-FluoSTEP-EKAR

   Plasmid

   #181858

   Purpose

   Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-EKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti.puro.MCV.ER.RAZ.2

   Plasmid

   #114382

   Purpose

   This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.

   Depositor

   Reety Arora, Sudhir Krishna

   Article

   Arora et al Microb Pathog. 2019 Dec;137:103778. doi:

   Insert

   MCV NCCR and tumor T antigen regions
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MCV NCCR (viral own promoter)

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1-HA-MEK1

   Plasmid

   #53159

   Purpose

   Expresses GST-HA tagged human MEK1 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-EphB2

   Plasmid

   #200982

   Purpose

   Mammalian expression plasmid for myc-tagged EphB2

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB2 (EPHB2 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKrox24(2xD-E_inD)Luc

   Plasmid

   #200109

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element in front of EGR1 promoter…

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MEF2A-Sensor

   Plasmid

   #208928

   Purpose

   in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   Mef2a docking sequence+Phospho site (MEF2A Human)
   

   Use

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-HA-MEK5

   Plasmid

   #53166

   Purpose

   Expresses GST-HA tagged human MEK5 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK5 (MAP2K5 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWZLblasti-HA-MEK1

   Plasmid

   #53161

   Purpose

   Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pWZLblasti-HA-MEK1 C121S

   Plasmid

   #53165

   Purpose

   Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

    Plasmid

    #123339

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_MAPK3_Pkinase

    Plasmid

    #110023

    Purpose

    Protein expression and purification of MAPK3_Pkinase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    MAPK3_Pkinase (MAPK3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

    Plasmid

    #123342

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX6P1-HA-MEK1 C121S

    Plasmid

    #53164

    Purpose

    Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    C121S

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pWZLblasti-HA-MEK5

    Plasmid

    #53167

    Purpose

    Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

    Plasmid

    #123341

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6P1-HA-MEK1 CBM

    Plasmid

    #53163

    Purpose

    Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    M187A/H188A/M230A/H239A

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGEX6P1-HA-MEK5 CBM-E

    Plasmid

    #53168

    Purpose

    Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    L280A/H281A/M323A/Q332A

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pWZLblasti-HA-MEK5 CBM-E

    Plasmid

    #53169

    Purpose

    Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L280A/H281A/M323A/Q332A

    Promoter

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pwM

    Plasmid

    #22515

    Depositor

    Christopher Buck

    Article

    Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

    Insert

    MCPyV VP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon modified gene

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ph2m

    Plasmid

    #22518

    Depositor

    Christopher Buck

    Article

    Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

    Insert

    MCPyV VP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon modified

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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