We narrowed to 1,280 results for: MS2

1. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MS2-p65-HSF1-P2A-mCherry

   Plasmid

   #184440

   Purpose

   MS2 coat protein fused with p65 and hsf1

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   MS2-p65-hsf1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPUR-hU6-sgRNA-Sirius-8XMS2

   Plasmid

   #121942

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   human U6 promoter

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEFS-ccdB-12X MS2_mPGK-Puro

   Plasmid

   #177809

   Purpose

   Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

   Type

   Empty backbone

   Tags

   12X MS2 stem loops

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

   Plasmid

   #86827

   Purpose

   Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   FLAG-NLS-MS2-eGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pDZ415 (24MS2SL loxP-Kan-loxP)

   Plasmid

   #45162

   Depositor

   Daniel Zenklusen, Robert Singer

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   24xMS2 Stem Loop Cassette
   

   Available Since

   Aug. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

   Plasmid

   #199442

   Purpose

   CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   HITI donor template
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

   Plasmid

   #192684

   Purpose

   Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-SMS2-TEV-Twin-Strep

   Plasmid

   #202529

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS2 (SGMS2 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TGA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. prDNA spacer-LacOx50-20xMS2-Hyg

    Plasmid

    #210219

    Purpose

    Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

    Plasmid

    #167313

    Purpose

    Template pDNA to prepare DD-CaVT mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    DD-CaVT
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

    Plasmid

    #219536

    Purpose

    a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLH-sgRNA-Sirius-4X(MS2-PP7)

    Plasmid

    #121941

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-4X(MS2-PP7)
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Gene Trap - 24XMS2 Lentiviral Vector

    Plasmid

    #174198

    Purpose

    Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

    Depositor

    Daniel Larson

    Article

    Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

    Insert

    iRFP
    

    Use

    Lentiviral

    Tags

    24X MS2 hairpins, Blasticidin, and T2A

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

    Plasmid

    #189792

    Purpose

    Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Mouse, Human)
    

    Expression

    Mammalian

    Promoter

    Ponasterone A inducible

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti pTERT sgRNA1(MS2) mCherry

    Plasmid

    #171054

    Purpose

    Targeting of Vp64-dCas9 activation system to hTERT promoter

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    pTERT sgRNA1(MS2) mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLH-sgRNA1-boxB-MS2-PP7

    Plasmid

    #75397

    Purpose

    sgRNA1-boxB-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-MS2V5-PABPC1 M161A/D165K

    Plasmid

    #65809

    Purpose

    expression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PABPC1 (PABPC3 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    M161A/D165K

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiU6-hMYOD1 gRNA_1-MS2-Puro

    Plasmid

    #192673

    Purpose

    Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNA #1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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