We narrowed to 1,196 results for: MS2;

1. pDZ415 (24MS2SL loxP-Kan-loxP)

   Plasmid

   #45162

   Depositor

   Robert Singer, Daniel Zenklusen

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   24xMS2 Stem Loop Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti pTERT sgRNA1(MS2) mCherry

   Plasmid

   #171054

   Purpose

   Targeting of Vp64-dCas9 activation system to hTERT promoter

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   pTERT sgRNA1(MS2) mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

   Plasmid

   #86827

   Purpose

   Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   FLAG-NLS-MS2-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pLH-sgRNA-Sirius-4X(MS2-PP7)

   Plasmid

   #121941

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-4X(MS2-PP7)
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiU6-hMYOD1 gRNA_1-MS2-Puro

   Plasmid

   #192673

   Purpose

   Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human MYOD1 activating gRNA #1 (MYOD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiU6-hASCL1 gRNA_1-MS2-Puro

   Plasmid

   #192670

   Purpose

   Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human ASCL1 activating gRNA #1 (ASCL1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-MS2V5-PABPC1 M161A/D165K

   Plasmid

   #65809

   Purpose

   expression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   PABPC1 (PABPC3 Human)
   

   Use

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   M161A/D165K

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLH-sgRNA1-boxB-MS2-PP7

   Plasmid

   #75397

   Purpose

   sgRNA1-boxB-MS2-PP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-boxB-MS2-PP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

    Plasmid

    #160627

    Purpose

    TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-MS2:TV-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

    Plasmid

    #219536

    Purpose

    a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

    Plasmid

    #160624

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2:VPR:Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

    Plasmid

    #136382

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

    Plasmid

    #147924

    Purpose

    Mammalian Expression of 6xbsMS2-Malat

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    6xbsMS2-Malat (Malat1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA(MS2)_zeo insert c-MYC

    Plasmid

    #164636

    Purpose

    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

    Depositor

    Günter Schneider

    Insert

    target sequence for c-Myc activation (MYC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS2CP(V29I)-myc-T2A-tagRFP

    Plasmid

    #140260

    Purpose

    Expresses MS2CP(V29I) in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

    Insert

    Coat protein of bacteriphage MS2
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti MS2-p65-HSF1 sfGFP

    Plasmid

    #171051

    Purpose

    Expression of Vp64-dCas9 activation system

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    MS2-p65-HSF1_sfGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192682

    Purpose

    Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEFS-ccdB-12X MS2_mPGK-Puro

    Plasmid

    #177809

    Purpose

    Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

    Type

    Empty backbone

    Use

    Tags

    12X MS2 stem loops

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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