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We narrowed to 977 results for: plasmids spcas9

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Showing: 461 - 480 of 977 results

  1. pLSC-5

    Plasmid
    #62889
    Purpose
    Lenti vector for expression of inducible split Cas9
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Inserts
    SpCas9(574-1368)
    SpCas9(2-573)
    Use
    CRISPR and Lentiviral
    Tags
    FFBP12, FRB, NES PTK2, NLS SV40, and P2A
    Expression
    Mammalian
    Mutation
    *see comment below
    Promoter
    EFS
    Available Since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62889-plasmid-map-sequence-id-105912

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  3. pCMV-eVRQR-P2A-EGFP (KAC1028)

    Plasmid
    #242663
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242663-plasmid-map-sequence-id-489032

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  4. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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  5. pITI1

    Plasmid
    #166094
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166094-plasmid-map-sequence-id-329610

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  6. pRR1

    Plasmid
    #166096
    Purpose
    Gal inducible expression of Cas9 WT
    Depositor
    Rebecca Burgess
    Insert
    WT spCas9
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Gal-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166096-plasmid-map-sequence-id-330354

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  7. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166088-plasmid-map-sequence-id-329112

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  8. pNSU299_SEO1

    Plasmid
    #166076
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SEO1 (SEO1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166076-plasmid-map-sequence-id-329607

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  9. DBJS-p2.25

    Plasmid
    #246894
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for UBAP2L Nterm.
    Depositor
    David Bartel
    Insert
    UBAP2L Nterm Guide RNA 1 (UBAP2L Human)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246894-plasmid-map-sequence-id-490581

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  10. pNSU299_APL3

    Plasmid
    #166073
    Purpose
    Plasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    APL3 (APL3 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166073-plasmid-map-sequence-id-329133

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  11. pNSU299_PPE1

    Plasmid
    #166083
    Purpose
    Plasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    PPE1 (PPE1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166083-plasmid-map-sequence-id-329122

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  18. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available Since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

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  19. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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Showing: 461 - 480 of 977 results