We narrowed to 25,015 results for: Nes

1. TET-pLKO.1 PURO shID2 #1

   Plasmid

   #83086

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human ID2

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pSLIK TT 3xFLAG ID2 neo

   Plasmid

   #98393

   Purpose

   Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   ID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe JunD-Venus puro

   Plasmid

   #58496

   Purpose

   mammalian expression of Venus-tagged mouse JunD

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   JunD (Jund Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

4. mCardinal-H3-23

   Plasmid

   #56163

   Purpose

   Localization: Nucleus/Histones, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   H3 (H3C2 Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pRC/CMV-TYK2K930R-FF-VSV

   Plasmid

   #139348

   Purpose

   Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop

   Depositor

   Sandra Pellegrini

   Article

   Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-K930R-Y1054F-Y1055F

   Promoter

   CMV

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC/CMV-TYK21054-5FF-VSV

   Plasmid

   #139347

   Purpose

   Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop

   Depositor

   Sandra Pellegrini

   Article

   Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V362F-Y1054F-Y1055F

   Promoter

   CMV

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSN-A112C-GpA-G83I

   Plasmid

   #191239

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
   

   Use

   Tags

   nuclease A from S. aureus fused to the TM domain …

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and Glycin…

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. tet-pLKO.1_puro_shJUND #1

   Plasmid

   #136581

   Purpose

   Expresses an inducible short hairpin targeting human JUND sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shJUNDv1 (JUND Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CSK_HUMAN_D0

   Plasmid

   #79683

   Purpose

   This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, Nicholas Levinson, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSK (CSK Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCHKU38.2-2.2

    Plasmid

    #110670

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRD439

    Plasmid

    #168465

    Purpose

    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mTurquoise2-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TTmiRc2_BirA_CDKN1A

    Plasmid

    #136523

    Purpose

    Used as a donor vector to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5 N539

    Plasmid

    #25972

    Depositor

    Rob Lewis

    Article

    Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted amino acids 540-873

    Promoter

    Available Since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCMV5 C540

    Plasmid

    #25971

    Depositor

    Rob Lewis

    Article

    Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletes amino acids 1-539

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pSLIK 3XFLAG-MKK7-EE-NLS hygro

    Plasmid

    #87780

    Purpose

    Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK7 (Map2k7 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pD10AH840AhCas9 (GB1041)

    Plasmid

    #68207

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGEN-DV-IRES-myr-mKate (JDW 494)

    Plasmid

    #242575

    Purpose

    Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-mKate to label cell membranes.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    attR1-CmR-ccdB-attR2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-HYGRO-P2A-mAID-mCLOVER-LRR1

    Plasmid

    #221546

    Purpose

    DONOR homology arm-containing repair template used to insert Auxin-Inducible Degron tags, mClover, and Hygromycin resistance markers to the N-terminus of LRR1

    Depositor

    Aga Gambus

    Article

    Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

    Insert

    LRR1 (LRR1 Human)
    

    Use

    Unspecified

    Tags

    HYGRO-P2A-mAID-mCLOVER

    Expression

    Mutation

    Promoter

    N/A

    Available Since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDF_AaRaf1/Raf2/RbcX2/BSD2/RbcX1

    Plasmid

    #229509

    Purpose

    Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco accumulation factor 1
    Rubisco accumulation factor 2
    RbcX2
    BSD2
    RbcX1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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