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pBACgus N-His/GST/LRRK2 1867-2186

Plasmid

#40403

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

WT aa1867-2186

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-URA3-3'SEC13-GFP

Plasmid

#25445

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

Insert

Sec13-Green Fluorescent Protein

Expression

Yeast

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAG416-GAL-FUS

Plasmid

#181711

Purpose

Galactose inducible expression of FUS

Depositor

Alejandro Chavez

Insert

FUS (FUS Human)

Expression

Yeast

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pTCW007

Plasmid

#83525

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha1

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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EZ-L439

Plasmid

#169146

Purpose

Encodes OptoINVRT7 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p413TEF-Sir2-H364Y

Plasmid

#51743

Purpose

overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Expression

Yeast

Mutation

H364Y

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pN3FS-CYC1

Plasmid

#85783

Purpose

N-ICE plasmid pH3FS-CYC1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-NatMX-loxP-CYC1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Mutation

*(see below)

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Sc eRF1 (WT)

Plasmid

#175413

Purpose

Encodes His-MBP-TEV-WT eRF1

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF1 (SUP45 Budding Yeast)

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Vrg4-V5-APEX2-URA

Plasmid

#207071

Purpose

Expressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Vrg4

Tags

V5-APEX2

Expression

Yeast

Promoter

pVRG4

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-4V5-APEX2-URA

Plasmid

#207063

Purpose

Backbone plasmid containing ADH3 promoter and 4V5-APEX2 tag.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Tgl3-4V5-APEX2-URA

Plasmid

#207066

Purpose

Expressing Tgl3-4V5-APEX2. Marker for lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Tags

FRB (T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMM0553

Plasmid

#128989

Purpose

LEU2 integration vector of pZF(3BS)-mRUBY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Leu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pXcX-3.6TPH-GAL4p65

Plasmid

#32573

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

Gal4p65

Use

Adenoviral

Expression

Mammalian

Promoter

3.6TPH

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-pol30(K164R)

Plasmid

#99544

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCHNFI-B2

Plasmid

#31405

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IB isoform 2 (Nfib Mouse)

Tags

HA

Expression

Mammalian

Mutation

Not the same as reference seq. See sequence for m…

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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GatA_pET29b

Plasmid

#215406

Purpose

Expression construct for GatA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

GatA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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MXS_TagBFP

Plasmid

#62400

Purpose

MXS_chaining vector with TagBFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

TagBFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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