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We narrowed to 14,904 results for: EGF

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Showing: 4801 - 4820 of 14904 results

  5. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid
    #187844
    Purpose
    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…
    Expression
    Mammalian
    Mutation
    meGFPe: asparagine 198 to aspartic acid and tyros…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187844-plasmid-map-sequence-id-379371

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  17. pLV-Enh eGFP Reporter-huKC2-IGR21

    Plasmid
    #59456
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the human keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    huKrt1 3’intergenic region
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191163
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP-3xMS2
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191163-plasmid-map-sequence-id-375409

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  20. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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Showing: 4801 - 4820 of 14904 results