We narrowed to 13,230 results for: CRISPR-Cas9

1. pX330A_FokI-1x4

   Plasmid

   #63604

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

   Plasmid

   #138318

   Purpose

   Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   SauCas9 GFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ159

   Plasmid

   #69308

   Purpose

   Gateway entry vector with hSpCas9D10A

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9D10A (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   D10A mutation; NLS fusion

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330A_FokI-1x7

   Plasmid

   #63607

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ158

   Plasmid

   #69307

   Purpose

   Gateway entry vector with hSpCas9 (Human codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9 (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   NLS fusion

   Promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_FokI-1x6

   Plasmid

   #63606

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF820_U6-sgRNA-EF1a-mCherry2_lenti

   Plasmid

   #138316

   Purpose

   Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   SpyCas9 GFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB1C3-hsg(M)

   Plasmid

   #138262

   Purpose

   Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   sgRNA M
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330A_FokI-1x3

   Plasmid

   #63603

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPEY-Z3-Kan

    Plasmid

    #232102

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPEY-Z3-Hyg

    Plasmid

    #232101

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS424-ysg(NT)

    Plasmid

    #138258

    Purpose

    Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB1C3-ysg(NT)

    Plasmid

    #138263

    Purpose

    Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA1_pNeurog2-Luciferase reporter

    Plasmid

    #64160

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for pNeurog2-Luciferase reporter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330A_FokI-1x5

    Plasmid

    #63605

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDB_268

    Plasmid

    #231112

    Purpose

    SpG-Cas9

    Depositor

    David Root, John Doench

    Insert

    Cas9-SpG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

    Plasmid

    #117136

    Purpose

    Empty vector encoding BFP-BSD

    Depositor

    Kosuke Yusa

    Insert

    BSD2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRNF219.1.0-gDNA

    Plasmid

    #132464

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RNF219
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUDP002

    Plasmid

    #103872

    Purpose

    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Inserts

    hph
    Spcas9 D147Y P411T
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Yeast

    Mutation

    D147Y P411T

    Promoter

    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. TC9pAB

    Plasmid

    #71310

    Purpose

    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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