We narrowed to 28,443 results for: Tat

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pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Pink

Plasmid

#160445

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-mLanYFP-polyA

Plasmid

#174021

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

mLanYFP

Use

Zebrafish knock-in tagging

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

Plasmid

#18017

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13V ECFP-W67G

Tags

ECFP-W67G and Venus

Expression

Mammalian

Mutation

mglB-D154A; non-functional ECFP

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCytERM-emiRFP670-N1

Plasmid

#197180

Purpose

Expresses the fusion protein of CytERM-emiRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-emiRFP670

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBluescript PMS1

Plasmid

#16457

Depositor

Bert Vogelstein

Article

Nicolaides et al Nature. 1994 Sep 1. 371(6492):75-80.

Insert

PMS1 (PMS1 Human)

Expression

Bacterial

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
Lyn-YFP-FRB1C

Plasmid

#162425

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-FRB1C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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FRB1N-CFP-CAAX

Plasmid

#162428

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

FRB1N-CFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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cp196V1.2

Plasmid

#52100

Purpose

A improved cpmCherry variant

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

cp196V1.2

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pTubulin-iRFP670

Plasmid

#197237

Purpose

Expresses the fusion protein of Tubulin-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-iRFP670

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP1C-CFP-Cb5

Plasmid

#162438

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

FKBP1C-CFP-Cb5

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Orange

Plasmid

#160444

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Orange

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mNeonGreen

Plasmid

#191323

Purpose

Expresses fluorescent labeled beta Tubulin to measure microtubule density

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mNeonGreen (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-SNIFP-N1

Plasmid

#197189

Purpose

Expresses the fusion protein of CytERM-SNIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-SNIFP

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

Plasmid

#18016

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13V Venus-Y67G

Tags

ECFP and Venus-Y67G

Expression

Mammalian

Mutation

mglB-D154A; non-functional Venus

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe-Kras G15A

Plasmid

#58898

Purpose

Expresses human Kras with a G15A mutation

Depositor

Channing Der

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

G15A mutation

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pETCON-DnvLB16

Plasmid

#45124

Depositor

David Baker

Article

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

DnvLB16

Tags

Aga2p for yeast surface display and c-myc epitope…

Expression

Yeast

Promoter

GAL

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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3xTRE DREaMR 3p3GFP

Plasmid

#182205

Purpose

3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-GFP

Expression

Insect

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCytERM-smURFP-N1

Plasmid

#197178

Purpose

Expresses the fusion protein of CytERM-smURFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-smURFP

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAS007

Plasmid

#164868

Purpose

This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.

Depositor

Keith Shearwin

Article

Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

Insert

phiKO2 operators

Use

Synthetic Biology

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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