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pDONR223-CAMKK1

Plasmid

#23824

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMKK1 (CAMKK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ITGB1BP3

Plasmid

#23393

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ITGB1BP3 (NMRK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RIOK2

Plasmid

#23535

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIOK2 (RIOK2 Human)

Use

Gateway Cloning

Mutation

C430T, T447A,T453A,1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CAMK1D

Plasmid

#23791

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMK1D (CAMK1D Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCAS

Plasmid

#206994

Purpose

Plasmid expressing both SpCas9 and SaCas9

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Insert

SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )

Tags

P2A

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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AZ64_pE.DonorCLYBL.TS

Plasmid

#199228

Purpose

Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

Expression unit for EGFP reporter

Use

Gene targeting donor plasmid

Expression

Mammalian

Promoter

Human PGK1 gene promoter

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_101

Plasmid

#160541

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-hPim1

Plasmid

#100722

Purpose

Expression of human Pim1 kinase domain in E.coli

Depositor

Byung Il Lee

Article

Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

Insert

Pim1 (PIM1 Human)

Tags

His

Expression

Bacterial

Mutation

Residues 29-313 of isoform 2

Promoter

T7 promotor

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1 CA

Plasmid

#79502

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

S127A

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-UHMK1

Plasmid

#23433

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

UHMK1 (UHMK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SH3BP5

Plasmid

#23579

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SH3BP5 (SH3BP5 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK32C

Plasmid

#23720

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK32C (STK32C Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

Plasmid

#204497

Purpose

Expresses rtTA and Neomycin resistance genes in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Insert

rtTA

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-StefinA

Plasmid

#182405

Purpose

Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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H2B-LSSmGFP

Plasmid

#186539

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Human Histone 2B (D26G and V119I mutations)

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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PRPF19

Plasmid

#155847

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF19 (PRPF19 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPIDCB2

Plasmid

#169900

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Expression

Mammalian

Promoter

Dox inducible

Available Since

Dec. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp3 Mac1 - P43 (Oxford)

Plasmid

#169210

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Article

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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