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pPN287

Plasmid

#91670

Purpose

Express sgRNA targeting human SLC45A1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SLC45A1 (SLC45A1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN196

Plasmid

#91641

Purpose

Express sgRNA targeting human MEF2C

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MEF2C (MEF2C Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN269

Plasmid

#91632

Purpose

Express sgRNA targeting human KCNB1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KCNB1 (KCNB1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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P21-sp64 Poly A

Plasmid

#59433

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 21

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_ARPM1

Plasmid

#45259

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

ARPM1 (ACTRT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_H2B-Rho-tag-T2A-mEGFP

Plasmid

#249667

Purpose

Co-expression of rhodamine-binding protein tag (nuclear) and mEGFP (not localized) in mammalian cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

H2B-Rho-tag-T2A-mEGFP

Expression

Mammalian

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC_Rho-tag-Pro30-mEGFP-NLS3x

Plasmid

#249671

Purpose

Expression of rhodamine-binding protein tag fused to mEGFP (nuclear) in zebrafish

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

Rho-tag-Pro30-mEGFP-NLS3x

Use

Zebrafish expression

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC_SiR-tag-Pro30-mEGFP-NLS3x

Plasmid

#249672

Purpose

Expression of silicon rhodamine-binding protein tag fused to mEGFP (nuclear) in zebrafish

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

SiR-tag-Pro30-mEGFP-NLS3x

Use

Zebrafish expression

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-SiR-tag-T2A-mEGFP

Plasmid

#249668

Purpose

Co-expression of silicon rhodamine-binding protein tag (nuclear) and mEGFP (not localized) in mammalian cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

H2B-SiR-tag-T2A-mEGFP

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pT7II-dGAEtau

Plasmid

#249558

Purpose

Recombinant protein synthesis of dGAE (AD Core) human Tau cysless variant.

Depositor

Jeff Kuret

Article

Pettis et al Anal Biochem. 2026 Mar 4;714:116095. doi

Insert

Tau (dGAE) (MAPT Human)

Expression

Bacterial

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GSDMD-Flag WT

Plasmid

#249726

Purpose

Express GSDMD WT in mammalian cells

Depositor

Hao Wu

Article

Du et al Nature. 2024 Jun;630(8016):437-446. doi:

Insert

gasdermin D (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pT7II-dGAE C322A

Plasmid

#249559

Purpose

Recombinant protein synthesis of dGAE (AD Core) human Tau (C322A mutant) cysless variant.

Depositor

Jeff Kuret

Article

Pettis et al Anal Biochem. 2026 Mar 4;714:116095. doi

Insert

Tau (dGAE C322A) (MAPT Human)

Expression

Bacterial

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pT7II-2N4Rtau I277P/I308P

Plasmid

#249557

Purpose

Recombinant protein synthesis of I277P/I308P mutant of human 2N4R Tau

Depositor

Jeff Kuret

Article

Pettis et al Anal Biochem. 2026 Mar 4;714:116095. doi

Insert

Tau (2N4R - I277P/I308P) (MAPT Human)

Expression

Bacterial

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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MBP_TS-B1AR_Trx_PreSc_NdeI_AgeI

Plasmid

#247768

Purpose

This plasmid is a modified version of pRG/III-hs-MBP (Addgene plasmid #121676), designed for the expression of a functional β1-adrenergic receptor (β1AR) in E. coli.

Depositor

Stephan Grzesiek

Article

Abiko et al J Biomol NMR. 2021 Jan;75(1):25-38. doi:

Insert

Thermostable Turkey B1-adrenergic receptor (TS-B1AR)

Tags

MBP–(GS)5–HRV 3C site–(GS)5–NdeI site_ B1AR_AgeI …

Expression

Bacterial

Promoter

lac promoter

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgYME1L1 sg1

Plasmid

#244867

Purpose

Knockout of human YME1L1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

YME1L1 sgRNA (YME1L1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SLC31A1_sg2

Plasmid

#244870

Purpose

Knockout of human SLC31A1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SLC31A1 sgRNA (SLC31A1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgYME1L1 sg2

Plasmid

#244868

Purpose

Knockout of human YME1L1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

YME1L1 sgRNA (YME1L1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SLC31A1_sg1

Plasmid

#244869

Purpose

Knockout of human SLC31A1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SLC31A1 sgRNA (SLC31A1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 UROS_sg1

Plasmid

#244873

Purpose

Knockout of human UROS

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

UROS sgRNA (UROS Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 UROS_sg2

Plasmid

#244874

Purpose

Knockout of human UROS

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

UROS sgRNA (UROS Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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