We narrowed to 8,227 results for: sars

1. pLenti-PGK-flag-RAP1B-V12-neo

   Plasmid

   #156175

   Purpose

   Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-flag-RAP1B-N17-neo

   Plasmid

   #156176

   Purpose

   Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3E-hGPR56 e1m promoter normal

   Plasmid

   #52298

   Purpose

   Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-RAP2A-N17-neo

   Plasmid

   #156179

   Purpose

   Lentiviral vector for expression of RAP2A-N17, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. shERK2b-mlpx-neo

   Plasmid

   #65231

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235)

   Plasmid

   #160646

   Purpose

   Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S, 35S

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.23 C3_9

   Plasmid

   #60295

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   NKX6-1 enhancer (NKX6-1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pSc1-puro

   Plasmid

   #80438

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   Puromycin resistance
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6, and hPGK

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-HES3

   Plasmid

   #101411

   Purpose

   Donor Vector containing HES3 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   HES3 (HES3 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVD1 tRNA-gRNA position E3 (GB2240)

    Plasmid

    #160562

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tRNA-gRNA position E3 (Multiplexing Edit)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL4.23 C3_29

    Plasmid

    #60309

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    ZFP36L2 enhancer (ZFP36L2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. XE129 beta2AR Fz1-pcDNA3.1

    Plasmid

    #16795

    Depositor

    Randall Moon

    Article

    Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

    Insert

    beta 2 AR Fz1 (Fzd1, Adrb2 Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Chimera of beta 2 adrenergic receptor (extracellu…

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pENTR D-TOPO-C3_10

    Plasmid

    #60259

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    RFX6 enhancer (RFX6 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR D-TOPO-C3_25

    Plasmid

    #60268

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    ROCK1 enhancer (ROCK1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4.23 C3_31

    Plasmid

    #60310

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    SLC2A2 enhancer (SLC2A2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pGL4.23 C3_21

    Plasmid

    #60296

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    CDKN2BAS enhancer (CDKN2B-AS1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGL4.23 C3_19

    Plasmid

    #60302

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    THADA enhancer (THADA Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

    Plasmid

    #52299

    Purpose

    Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    15-bp deletion in the promoter

    Promoter

    Human GPR56 e1m promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 FFSS-BACH1 C122E

    Plasmid

    #232274

    Purpose

    Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    changed cysteine 122 to glutamic acid

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304-BCL-XL-499/500R

    Plasmid

    #203575

    Purpose

    Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    silent point mutations to make resistant shRNA 49…

    Promoter

    CMV

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. sh2g-ZMYND8-human

    Plasmid

    #201406

    Purpose

    Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

    Insert

    ZMYND8 (ZMYND8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. sh4p-ZMYND8-mouse

    Plasmid

    #201405

    Purpose

    Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

    Insert

    ZMYND8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1+HIF1a-AS1

    Plasmid

    #194172

    Purpose

    Expresses lncRNA HIF1a-AS1 (spliced)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1+HIF1a-AS1_deltaExon2

    Plasmid

    #194173

    Purpose

    Expresses lncRNA HIF1a-AS1 Exon1

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Exon2 was deleted

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1+HIF1a-AS1_deltaExon1

    Plasmid

    #194174

    Purpose

    Expresses lncRNA HIF1a-AS1 Exon2

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Exon1 was deleted

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

    Plasmid

    #194175

    Purpose

    Expresses lncRNA HIF1a-AS1 mutant

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Within Exon1, nucleotides 26-78 were removed

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1+HIF1a-AS1_1200_unspliced

    Plasmid

    #194176

    Purpose

    Expresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV6-MPP8-W80A-10xHis

    Plasmid

    #194179

    Purpose

    Expresses MPP8-W80A_10xHis

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-W80A inside chromo domain

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV6-MPP8-delta55-118-10xHis

    Plasmid

    #194180

    Purpose

    Expresses MPP8-delta55-118_10xHis

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-delta55-118 (chromo domain deletion)

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV6-MPP8-delta2-441-10xHis

    Plasmid

    #194181

    Purpose

    Expresses MPP8-delta2-441_10xHis

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-delta2-441

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge