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pPKm-241

Plasmid

#104855

Purpose

pSIN-cytosFD-P2A-cytosFNR, expressing Synechococcus sp Fd and FNR, under EF-1alpha promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

cytosFd-cytosFNR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Availability

Academic Institutions and Nonprofits only

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pPKm-292

Plasmid

#105816

Purpose

pcDNA - GAL4 DBD - MTAD. Expresses Gal4 DNA binding domain fused to MTAD

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

GAL4 DBD and MTAD

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 L306P

Plasmid

#169262

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 L306P (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu306Pro)

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 dE5

Plasmid

#169263

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E5 (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 dE8

Plasmid

#169264

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E8 (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 L351P

Plasmid

#169266

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 L351P (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu351Pro)

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE5

Plasmid

#169267

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E5 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE8

Plasmid

#169268

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E8 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E5 GT>A minigene

Plasmid

#169270

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 GT>A minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 GT>A minigene (RBM28 Human)

Expression

Mammalian

Mutation

NM_018077.2:c.(541+1_541+2delinsA)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E8 G>T minigene

Plasmid

#169272

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 G>T minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 G>T minigene (RBM28 Human)

Expression

Mammalian

Mutation

NM_018077.2:c.(946G>T)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

Plasmid

#169273

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 Exon1-6 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

Plasmid

#169274

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 Exon1-6 GT>A minigene (RBM28 Human)

Expression

Mammalian

Mutation

NM_018077.2:c.(541+1_541+2delinsA)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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HDM_omicron_B11529_IDTDNA

Plasmid

#204142

Purpose

Expression plasmid for SARS-CoV-2 BA.1 variant spike

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

SARS-CoV-2 BA.1 variant spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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FUW mCherry-GFP-LC3

Plasmid

#110060

Purpose

To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

Depositor

Anne Brunet

Article

Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

Insert

mCherry-GFP-LC3

Use

Lentiviral

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P REV3L_1

Plasmid

#160797

Purpose

Suppress REV3L

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shREV3L_1

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DNA2_2

Plasmid

#160780

Purpose

Suppress DNA2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDNA2_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DNA2_1

Plasmid

#160779

Purpose

Suppress DNA2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDNA2_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

Plasmid

#233036

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and hfCas13d

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapErk)DsRed

Plasmid

#200114

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence, no other pr…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159816

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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