We narrowed to 15,374 results for: NOL

1. 7xNLS-pMJ915v2-sfGFP

   Plasmid

   #88922

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST1374-Lb-Cpf1-NLS

   Plasmid

   #107680

   Purpose

   Expresses LbCpf1 in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   LbCpf1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB4132

   Plasmid

   #106127

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL2B -688/+64

   Plasmid

   #24944

   Depositor

   Stephen Smale

   Article

   Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

   Insert

   IL-10 promoter (Il10 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pGL2B -78/+64

   Plasmid

   #24947

   Depositor

   Stephen Smale

   Article

   Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

   Insert

   IL-10 promoter (Il10 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pET20b- hTPI Lys13Arg

   Plasmid

   #50726

   Purpose

   overexpression of the human TPI1 Lys13Arg allele in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   changed Lysine 13 to Argenine

   Promoter

   T7

   Available since

   Feb. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCellFree_G03 S100A1

   Plasmid

   #67048

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   S100A1 (S100A1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCellFree_G06

   Plasmid

   #67140

   Purpose

   Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   HIS and mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS-TarHom-Tol2-FRT-GalK-cryaa-YFP

   Plasmid

   #73198

   Purpose

   BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Citrine

   Depositor

   Holger Knaut

   Article

   Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

   Insert

   YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCKan-yibDp-GST

    Plasmid

    #134592

    Purpose

    Low Phosphate inducible GST

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Glutathione S-transferase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBT362

    Plasmid

    #127844

    Purpose

    clone BE4max-NG CBEs by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    BE4max-NG without deaminase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6Rosa-CAG-Cas9

    Plasmid

    #118998

    Purpose

    Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMK388 (mClover-BSD)

    Plasmid

    #121189

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mClover-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-8xHis

    Plasmid

    #101699

    Purpose

    8xHis in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    AATG-[8xHis]-AGCC
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. V957 pLPS-3' ECFP-mito - Acceptor

    Plasmid

    #11699

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Acceptor vector

    Tags

    ECFP-mito

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCellFree_G09

    Plasmid

    #67143

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis sfGFP tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His and sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330A_FokI-1x4

    Plasmid

    #63604

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTMS2

    Plasmid

    #87800

    Purpose

    For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TAL2368

    Plasmid

    #36779

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR1-TALEN_Left (Ncor1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCfB5935

    Plasmid

    #106128

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge