We narrowed to 11,635 results for: Vars

1. pCWXPGR-pTF-Wnt3a

   Plasmid

   #114287

   Purpose

   TET-inducible expression of Wnt3a

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   Wnt3a (Wnt3a Mouse)
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAT077

   Plasmid

   #180511

   Purpose

   Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   PlmCasX
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-eNme2-C-BE4max

   Plasmid

   #183679

   Purpose

   Expresses eNme2-C-BE4 in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   rAPOBEC1-neNme2-C-2xUGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-MPRA-MluI-SpeI-EcoRI

   Plasmid

   #190196

   Purpose

   Empty vector for inserting MPRA libraries into AAV

   Depositor

   Hyejung Won

   Article

   McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

   Type

   Empty backbone

   Use

   AAV; Massively parallel reporter assay (mpra)

   Expression

   Mammalian

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK341

   Plasmid

   #192640

   Purpose

   Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpRY
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   SoxS has R93A and S101A mutations and dSpRY has s…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_GAS7-1/1

   Plasmid

   #91301

   Purpose

   Protein expression and purification of human SH3 domain construct GAS7-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   GAS7-1/1 (GAS7 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rat Alpha2 delta-1 HA pMT2

   Plasmid

   #58729

   Purpose

   expression of HA tagged rat Alpha2 delta-1 in mamalian cells

   Depositor

   Annette Dolphin

   Article

   Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

   Insert

   alpha2 delta-1 (Cacna2d1 Rat)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

   Plasmid

   #170248

   Purpose

   Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Mutation

   C124S

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHA

   Plasmid

   #194382

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   ARRB2-CTEV-2xHA (ARRB2 Synthetic, Human)
   

   Tags

   2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2/sh-Col1a1/GFP4_Seq1.2

    Plasmid

    #201403

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Expression

    Mammalian

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFR G719S

    Plasmid

    #11013

    Purpose

    Retroviral construct for expressing human EGFR with G719S mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR G719S (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G719S

    Available Since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

    Plasmid

    #159817

    Purpose

    Positive control for germline GFP expression from simple array in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV_EF1a_CCNB1-P2A-Hygro_Barcode

    Plasmid

    #170218

    Purpose

    Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CCNB1 (CCNB1 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-CreERT2 sgRipk4 v3

    Plasmid

    #158048

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Ripk4 sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLKO-CreERT2 sgAdam10 v3

    Plasmid

    #158047

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Adam10 sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pUCmini-iCAP-PHP.B3

    Plasmid

    #103004

    Purpose

    non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B3 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCmini-iCAP-PHP.B2

    Plasmid

    #103003

    Purpose

    non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B2 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHBS1449 SED1-Lenti

    Plasmid

    #180824

    Purpose

    SED1 biosensor for stable integration and expression in mammalian cells

    Depositor

    Rajat Rohatgi

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 osmosensor
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-HisMBP-SED1

    Plasmid

    #178191

    Purpose

    SED1 biosensor for expression in Escherichia coli

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1P CISD1_1

    Plasmid

    #160773

    Purpose

    Suppress CISD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shCISD1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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