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pBabe EGFR Del1(T790M)

Plasmid

#32072

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete E746-A750, T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e

Plasmid

#193645

Purpose

Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

Plasmid

#167295

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAK (BAK1 Human)

Expression

Mammalian

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 P-cad

Plasmid

#47502

Purpose

Expresses P-cadherin in mammalian cells

Depositor

Alice Wong

Article

Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

Insert

cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRKC-V5/HIS

Plasmid

#205615

Purpose

Expression of human TRKC receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TRKC receptor tyrosine kinase (NTRK3 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry N1 TOMM20

Plasmid

#157761

Purpose

Expression of TOMM20:mcherry in cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

TOMM20 (TOMM20 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 RDF ( GB1498)

Plasmid

#160579

Purpose

Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapErk)dTomato

Plasmid

#200113

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence, no other pr…

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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hCCR5-mTFP1

Plasmid

#110193

Purpose

Encodes for human CCR5 coding sequence tagged with the mTFP1 FP

Depositor

Sergi Padilla Parra

Article

Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

Insert

hCCR5 (CCR5 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RET-V5/HIS

Plasmid

#205606

Purpose

Expression of human RET receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

RET receptor tyrosine kinase (RET Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2022

Plasmid

#49129

Purpose

AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-PRS2/3:Con-iCre

Use

AAV

Expression

Mammalian

Promoter

DBH

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pKrox24(2xD-E)dTomato

Plasmid

#200110

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element, no other promoter elemen…

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRR.1

Plasmid

#69995

Purpose

Mammalian expression of cytochrome p450 CYP2B6

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry C1 MFF

Plasmid

#157760

Purpose

Expression of mcherry:MFF in cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

MFF (MFF Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

Plasmid

#167296

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAX (BAX Human)

Expression

Mammalian

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS_L_MARV

Plasmid

#135473

Purpose

Mammalian expression vector encoding Marburg virus L gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

L gene

Expression

Mammalian

Mutation

silent mutation (C to T) at position 7861; insert…

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-E2

Plasmid

#194590

Purpose

Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

E2 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-F1

Plasmid

#194592

Purpose

Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

F1 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-AA-GFP

Plasmid

#187259

Purpose

Lentiviral expression of myosin regulatory light chain-AA mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Mutation

T18A,S19A

Promoter

5' LTR

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV25

Plasmid

#237982

Purpose

Encodes TRAV25 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV25 (TRAV25 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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