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KCNE1_pCSdest

Plasmid

#53871

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

KCNE1 (KCNE1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
p5064 pcDNA3.1/V5-His-TOPO hBrd4 (1-461)

Plasmid

#14443

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-461) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1-461

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
CRYZL1_pCSdest

Plasmid

#53882

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CRYZL1 (CRYZL1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
Flag-NHR2+R pcDNA3.1

Plasmid

#17317

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
BTG3_pCSdest

Plasmid

#53865

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

BTG3 (BTG3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDECKO-mCherry MALAT1_Exon.2

Plasmid

#78541

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.4

Plasmid

#78543

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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NHR1+2-Flag pcDNA3.1

Plasmid

#17316

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCR3-vsv-CenpB 1-166-Sgo1 N61I

Plasmid

#108498

Purpose

expression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1, no PP2A binding N…

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRVH1-hygro shStx3

Plasmid

#40071

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Stx3 shRNA-1

Use

Retroviral

Promoter

H1

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
tdEos-PMP-N-10

Plasmid

#57658

Purpose

Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

Insert

PMP (PXMP2 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
NHR1-Flag pcDNA3.1

Plasmid

#17315

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
Neurl1-FLAG/Jag1-HA pIRES

Plasmid

#17337

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
Neurl1G2A-Flag pcDNA3.1

Plasmid

#17320

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Gly 2 changed to Ala

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pTarget_dnaA-mEos4b

Plasmid

#212607

Purpose

Contains the E. coli codon-optimised gene of the photoactivatable fluorescent protein mEos4b, together with a sgRNA for SpydCas9 targeting the E. coli dnaA gene.

Depositor

John van der Oost

Article

Olivi et al Nat Commun. 2025 Aug 21;16(1):7813. doi:

Insert

mEos4b

Use

CRISPR

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pTarget_dnaA-PAmCherry2.1

Plasmid

#212605

Purpose

Contains the E. coli codon-optimised gene of the photoactivatable fluorescent protein PAmCherry2(M10L), together with a sgRNA for SpydCas9 targeting the E. coli dnaA gene.

Depositor

John van der Oost

Article

Olivi et al Nat Commun. 2025 Aug 21;16(1):7813. doi:

Insert

PAmCherry2.1

Use

CRISPR

Mutation

M10L

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEndo-I-OnuI-TSM

Plasmid

#207954

Purpose

Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.

Depositor

David Edgell

Article

Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

Insert

Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.

Use

Synthetic Biology

Expression

Bacterial

Mutation

Leucine 212 changed to proline, leucine 434 in fu…

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5

Plasmid

#166163

Purpose

Gateway entry vector for N. furzeriDDX5

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-DDX5(kf)-SNAP

Plasmid

#166152

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Tags

His10 and SNAP

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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