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pHIS3p:CloverGFP-Tub1+3'UTR::HIS3

Plasmid

#50654

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

CloverGFP-Tub1

Use

Yeast expression

Tags

CloverGFP

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-humanTRAF3 (1098)

Plasmid

#66927

Purpose

Mammalian Expression of TRAF3

Depositor

Michael Karin

Insert

TRAF3 (TRAF3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Cas12aUltra_5XNLS

Plasmid

#218775

Purpose

His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

Depositor

Ross Wilson

Article

Sahu et al Nat Protoc. 2025 Mar 3. doi: 10.1038/s41

Insert

5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a

Use

CRISPR

Tags

6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

Expression

Bacterial

Mutation

Nucleotides mutations were made to encode for arg…

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR_TRAC

Plasmid

#164993

Purpose

Expression of gRNA targeting TCRalpha constant locus

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against TRAC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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BUN21/pML300 (recipient strain)

Bacterial Strain

#11675

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Bacterial Resistance

Spectinomycin

Species

Bacteriophage lambda

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
pYPQ220-Ultra

Plasmid

#176889

Purpose

Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

Insert

AsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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sgTrack-BFP

Plasmid

#164273

Purpose

Empty sgRNA expression vector with co-expression of BFP reporter

Depositor

Charles Rudin

Article

Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EFS

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-TALEN1

Plasmid

#83368

Purpose

Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN1

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-TRAIL-R4

Plasmid

#61385

Purpose

Expresses HA tagged TRAIL receptor 4

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 4 (TNFRSF10D Human)

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R4 leader peptide (aa1-29) replaced with TN…

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pTracer-EF-mFxh

Plasmid

#21927

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2006 Feb . 26(4):1209-22.

Insert

Fxh (Rbfox2 Mouse)

Tags

His and V5

Expression

Mammalian

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-DM-TRAF

Plasmid

#16377

Depositor

Wafik El-Deiry

Article

Sax et al J Biol Chem. 2003 Sep 19. 278(38):36435-

Insert

TRAF4 (TRAF4 Human)

Expression

Mammalian

Mutation

TRAF domain deletion mutant

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
TRAPPC8_pLX307

Plasmid

#98375

Purpose

Lentiviral expression of TRAPPC8

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TRAPPC8 (TRAPPC8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_263

Plasmid

#91127

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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JM110

Bacterial Strain

#49763

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

Streptomycin

Available Since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only
pHIS3p:mTurquoise2-Tub1+3'UTR::HPH

Plasmid

#50629

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mTurquoise2-Tub1

Use

Yeast expression

Tags

mTurquoise2

Promoter

HIS3p

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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Syn61Δ3(ev5)

Bacterial Strain

#174514

Purpose

Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Bacterial Resistance

Streptomycin

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only
pLY59_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2(stop)-TRAC-RHA(3166mut)

Plasmid

#192189

Purpose

CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059) (CD22 Synthetic)

Use

AAV; Mammalian expression

Mutation

NA

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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