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pmScarlet-H_C1

Plasmid

#85043

Purpose

Mammalian expression of mScarlet-H

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Lyn-tailed mCherry-SEpHluorin

Plasmid

#32002

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

Membrane-targeting sequence of Lyn kinase

Expression

Mammalian

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102G

Plasmid

#112176

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Expression

Mammalian

Mutation

F102G

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL-Vinculin-Venus

Plasmid

#111833

Purpose

Vinculin (chicken) tagged with VenusA206K at the C-terminus in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

Vinculin-Venus (VCL Chicken)

Use

Lentiviral

Tags

VenusA206K

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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VinculinTS-A50I

Plasmid

#111827

Purpose

Vinculin tension sensor with point mutation affecting ability to bind talin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (A50I)

Expression

Mammalian

Mutation

changed alanine 50 to isoleucine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102Y.Y137L

Plasmid

#112177

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Expression

Mammalian

Mutation

F102Y.Y137L

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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p5E mpeg1

Plasmid

#135199

Purpose

Macrophage specific label

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mpeg1 promoter

Use

Synthetic Biology

Promoter

mpeg1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-mEm

Plasmid

#185596

Purpose

Lentiviral construct for expression of mEmerald targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-mEm

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfGFPBb

Plasmid

#118231

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-butelase1

Plasmid

#115430

Purpose

Expresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coli

Depositor

Joshua Mylne

Article

James et al Plant J. 2019 Feb 21. doi: 10.1111/tpj.1

Insert

butelase 1

Tags

6-His

Promoter

T5

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ Dkk1-flag

Plasmid

#16690

Depositor

Xi He

Article

Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Insert

Dkk1 (DKK1 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO-cell-mCer

Plasmid

#185601

Purpose

Lentiviral construct for expression of mCerulean

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

cell-mCer

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

CAGG

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pEM351

Plasmid

#248763

Purpose

80xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-80xTetO

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEM414

Plasmid

#248766

Purpose

20xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-20xTetO

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pPP444

Plasmid

#158802

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRIP3-mCherry

Plasmid

#61386

Purpose

Expresses RIP3-mCherry in mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

RIP3 (RIPK3 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 S6K2 WT

Plasmid

#17729

Depositor

John Blenis

Article

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Insert

S6K2 (RPS6KB2 Human)

Tags

HA

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pLKO-cell-mEm

Plasmid

#185595

Purpose

Lentiviral construct for expression of mEmerald

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

cell-mEm

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pPP089

Plasmid

#158796

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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