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pDONR223_U2AF1_WT

Plasmid

#81807

Purpose

Gateway Donor vector containing U2AF1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

U2AF1 (U2AF1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.E47A

Plasmid

#81645

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

E47A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PDCD1LG2_WT

Plasmid

#82287

Purpose

Gateway Donor vector containing PDCD1LG2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PDCD1LG2 (PDCD1LG2 Human)

Use

Gateway entry vector

Mutation

F229S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-RnACE2 (Rat)

Plasmid

#158086

Purpose

Expresses rat ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Rat ACE2 (Ace2 Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.K134N

Plasmid

#81647

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

K134N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-NONO 40

Plasmid

#127653

Purpose

Knock-out of human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO sgRNA (NONO Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-NONO 4

Plasmid

#127652

Purpose

Knock-out of human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO sgRNA (NONO Human)

Use

Lentiviral

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNFAIP3_WT

Plasmid

#82238

Purpose

Gateway Donor vector containing TNFAIP3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFAIP3 (TNFAIP3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-FcACE2 (Cat)

Plasmid

#158082

Purpose

Expresses cat ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Cat ACE2 (ACE2 )

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRDM1_WT

Plasmid

#81740

Purpose

Gateway Donor vector containing PRDM1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRDM1 (PRDM1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.SUZ12

Plasmid

#125172

Purpose

Expresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

SUZ12 (SUZ12 Human)

Tags

His-TEV

Expression

Insect

Promoter

Polyhedrin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_WT

Plasmid

#81744

Purpose

Gateway Donor vector containing STK11 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJV136

Plasmid

#124255

Purpose

Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 9

Depositor

Stanley Riddell

Article

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (KRAS Human)

Use

Lentiviral

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_WT

Plasmid

#81771

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_POT1_WT

Plasmid

#81820

Purpose

Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

POT1 (POT1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PNKP_WT

Plasmid

#81801

Purpose

Gateway Donor vector containing PNKP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PNKP (PNKP Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET30-2-PSPH

Plasmid

#83909

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine phosphatase (PSPH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_TUBB_WT

Plasmid

#81990

Purpose

Gateway Donor vector containing TUBB, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TUBB (TUBB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKACB_WT

Plasmid

#82224

Purpose

Gateway Donor vector containing PRKACB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKACB (PRKACB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBS L30 Flag-Apex-Rab7A

Plasmid

#135652

Purpose

Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Tags

Apex2 and FLag

Expression

Mammalian

Promoter

L30

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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