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Plasmid#180619PurposeBacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.DepositorInsertKATNAL2 (KATNAL2 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-98PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 RPS6KC1 MIT, residues 235-316
Plasmid#180622PurposeBacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.DepositorInsertRPS6KC1 (RPS6KC1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 235-316PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b K294R
Plasmid#118359PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationK294RPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S307A
Plasmid#118364PurposeThis plasmid expresses human HSF1 S307A mutant.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS307APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)
Plasmid#199605PurposeExpression of cell permeable opAsCas12a in E. Coli.DepositorInsertopCas12a-T8N
UseCRISPRTags6xHis, Twin-strep, SUMOExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3
Plasmid#89975PurposeExpresses GFP-tagged ataxin-3DepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303/7A
Plasmid#118365PurposeThis plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303A and S307APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-pcDNA3.1
Plasmid#85760Purposemammalian expression of full length HA tagged USPL1DepositorInsertUSPL1, full length (USPL1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pETM30
Plasmid#85768Purposebacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
UseTagsGST and HisExpressionBacterialMutationcatalytic domain (aa 212-514)PromoterT7-lacAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
hGATA-1 K137R
Plasmid#118353PurposeThis plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.DepositorInserthGATA-1 (GATA1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK137RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K298R
Plasmid#118362PurposeThis plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK298RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pEYFP_C1
Plasmid#85766Purposemammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
UseTagsEYFPExpressionMammalianMutationcatalytic domain (aa 212-514)PromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-UBC9-sh4
Plasmid#168988PurposeExpresses RFP cDNA and UBC9 shRNA 4 in mammalian cellsDepositorInsertUbiquitin Conjugating Enzyme 9
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303A
Plasmid#118363PurposeThis plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
NP-11-63
Plasmid#234358PurposeExpressing and purifying R.rubrum rubisco using a his-sumo tagDepositorInsertR.rubrum rubisco
UseSynthetic BiologyTagsN-term SUMO tagExpressionMutationPromoterT7Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 delta-SLD1
Plasmid#210264PurposeInducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.DepositorInsertNIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationdelta-SLD1PromoterCMV/TetO2Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 D394R
Plasmid#210265PurposeInducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 D394R siRES (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationD394R, siRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 delta-N1
Plasmid#210268PurposeInducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 delta-N1 (aa 208-419) siRES (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationdelta-N1, siRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 delta-N2
Plasmid#210269PurposeInducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 delta-N2 (aa 261-419) siRES (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationdelta-N2, siRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NIP45 D394R
Plasmid#210267PurposeInducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 D394R siRES (NFATC2IP Synthetic)
UseTagsmCherryExpressionMammalianMutationD394R, siRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NIP45 wt
Plasmid#210266PurposeInducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
UseTagsmCherryExpressionMammalianMutationsiRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 delta-SLD2
Plasmid#210263PurposeInducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.DepositorInsertNIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationdelta-SLD2PromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 wt
Plasmid#210262PurposeInducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationsiRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C, residues 156-233, no Cys
Plasmid#180642PurposeBacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.DepositorInsertCHMP4C (CHMP4C Human)
UseTagsHIS-SUMOExpressionBacterialMutationresidues 316-366PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1, residues 315-360, no Cys
Plasmid#180643PurposeBacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.DepositorInsertIST1 (IST1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationresidues 315-360PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNA1 MIT residues 1-79
Plasmid#180614PurposeBacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.DepositorInsertKATNA1 (KATNA1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-79PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNA1 MIT residues 1-79, V55D
Plasmid#180615PurposeBacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.DepositorInsertKATNA1 (KATNA1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-79, V55D mutationPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL1 MIT residues 1-87
Plasmid#180618PurposeBacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.DepositorInsertKATNAL1 (KATNAL1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-87PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1B 4-199
Plasmid#115325PurposeExpresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23DepositorInsertCHMP1B (CHMP1B Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1B 1-199
Plasmid#115326PurposeExpresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24DepositorInsertCHMP1B (CHMP1B Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only