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Plasmid#18786DepositorInsertTRAP binding site
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-Tmprss6(S762A)
Plasmid#18791DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTags3xFLAGExpressionMammalianMutationChanged Serine762 to AlaninePromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-AHPH
Plasmid#187278PurposeExpress mCherry-AHPH (RhoA biosensor)DepositorInsertAnillin and Pleckstrin homology domain (ANLN Human)
UseTagsmCherryExpressionMammalianMutationAHPHPromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
IL2-mCherry in pmCherryN1
Plasmid#187283PurposeExpress IL2-mCherryDepositorInsertinterleukin-2 receptor alpha-subunit (IL2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
IL2-AHDC2-Cherry in pmCherryN1
Plasmid#187291PurposeExpress pmCherry-IL2-AH-deltaC2DepositorUseTagsmCherryExpressionMammalianMutationAH-deltaC2PromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTripZ-Tet-ON mCherry
Plasmid#187262PurposeDoxycyclin inducible expression of mCherryDepositorInsertmCherry-NLS
UseLentiviralTagsmCherryExpressionMutationPromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-MRLC-WT-GFP
Plasmid#187258PurposeLentiviral expression of myosin regulatory light chain-WTDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsEGFPExpressionMutationPromoter5' LTRAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 shCavin1KDR-EGFP
Plasmid#187254PurposeLentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)DepositorInsertShRNA for Cavin1 (Cavin1 Mouse)
UseLentiviralTagsEGFPExpressionMutationPromoterU6, 5'LTRAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_ΔC2 (157)
Plasmid#187720PurposeExpress GST-tagged NEDD4L ΔC2 (157) in insect cellsDepositorInsertNEDD4L residues 157-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-156PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_W2-W3-W4-HECT (349)
Plasmid#187721PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cellsDepositorInsertNEDD4L residues 349-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-348PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_FL
Plasmid#187722PurposeExpress His-tagged NEDD4L FL in insect cellsDepositorInsertNEDD4L FL (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT
Plasmid#187723PurposeExpress His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cellsDepositorInsertNEDD4L residues 1-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_W1-L-W2-HECT
Plasmid#187724PurposeExpress His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cellsDepositorInsertNEDD4L residues 153-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 1-152 and 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (381-975)_pcDNA3.1(+)
Plasmid#187725PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-380PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (492-975)_pcDNA3.1(+)
Plasmid#187726PurposeExpress his-tagged NEDD4L W3-W4-HECT in mammalian cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-491PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)
Plasmid#187727PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 226-382PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)
Plasmid#187728PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 418-495PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_C942S_pCDNA3.1(+)
Plasmid#187729PurposeExpress the catalytically inactive NEDD4L FL in mammalian cellsDepositorInsertNEDD4L FL with C874 mutated to a serine (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationCys874 mutated to a serinePromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_FL_pCDNA3.1(+)
Plasmid#187730PurposeExpress NEDD4L FL in mammalian cellsDepositorInsertNEDD4L FL (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996
Plasmid#187732PurposeExpress a chimera of the Nav1.5 linker between the first and second transmembrane domain with the motif surrounding PPSY; residues 1974-1977 PPSY mutated to AASADepositorInsertNav1.5 1,2linker residues 411-717 and Nav1.5 residues 1960-1996 PPSY mutated to AASA (SCN5A Human)
UseTagsHis-TEVExpressionBacterialMutationdeletion of residues 1-410 and 718-1959 and 1997-…PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only