Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 2,961 results for: COB;

Showing: 481 - 500 of 2961 results

  4. pEG302 5aa SunTag VP64 gRNA4 (FWA)

    Plasmid
    #115481
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    115481-plasmid-map-sequence-id-241913

    Enlarge

  8. pEG302 5aa SunTag VP64 gRNA17 (FWA)

    Plasmid
    #115482
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115482-plasmid-map-sequence-id-238819

    Enlarge

  15. pYUBcLIC-GFP

    Plasmid
    #183509
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    183509-plasmid-map-sequence-id-362497

    Enlarge

  16. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197958
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CmYLCVp and pUBQ10
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197958-plasmid-map-sequence-id-387866

    Enlarge

  18. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid
    #190174
    Purpose
    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway
    Depositor
    Gary Rudnick
    Article
    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.
    Insert
    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    Use
    Tags
    FLAG, His6, and c-myc
    Expression
    Mammalian
    Mutation
    changed Cys109 to alanine, changed tyr107 to cyst…
    Promoter
    CMV
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190174-plasmid-map-sequence-id-377202

    Enlarge

Showing: 481 - 500 of 2961 results