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Plasmid#183377PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαWT-IRES-EGFP
Plasmid#183456PurposeReplacement of endogenous rat PKA-RIIα subunits with wild-type RIIαDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralTagsExpressionMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B IRES GFP
Plasmid#133817PurposeEncodes the C2B domain of synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcna1a-IRES-EGFP
Plasmid#164965PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFPDepositorInsertkcna1a (kcna1a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-pax3a-kcnj13-IRES-EGFP
Plasmid#164950PurposeTol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoterpax3a promoter -5.4kAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-IRES-EGFP
Plasmid#164941PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Drd1a->M71-IRES-tauGFP ACNF TV
Plasmid#105073PurposeTargeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassetteDepositorUseMouse TargetingTagsExpressionMutationDrd1a coding sequence followed by IRES-tauGFP ACNFPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
HD201: pMVP (L3-L2) IRES-eGFP + polyA
Plasmid#121747PurposepMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.DepositorInsertIRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-AICD-NES-IRES-hrGFP
Plasmid#107544PurposeAAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFPDepositorInsertAICD-NES (APP Human)
UseAAVTagsExpressionMutationNonePromoterCMVAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-S75F
Plasmid#24580DepositorInsertOfd1 (Ofd1 Mouse)
UseTagsMyc and eGFPExpressionMammalianMutationserine 75 changed to phenylalanine (codon TCT cha…PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/UAA-mEGFP-IRES-mCherry
Plasmid#49234PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A IRES GFP
Plasmid#133818PurposeEncodes the C2A domain of synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV T274V KSR1 IRES GFP
Plasmid#25977DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged threonine 274 to valinePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV S392A KSR1 IRES GFP
Plasmid#25978DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged serine 392 to alaninePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
Mc4r->M71-IRES-tauGFP ACNF TV
Plasmid#105072PurposeTargeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-333 of Mc4r and an IRES-tauGFP followed by ACNF cassetteDepositorInsertMc4r->M71-IRES-tauGFP-ACNF (Mc4r Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV mouse Vav1DN IRES GFP
Plasmid#107092PurposeMammalian expression retroviral vectorDepositorInsertVav1 dominant negative cDNA (Vav1 Mouse)
UseRetroviralTagsExpressionMammalianMutationAA 1-186PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-A80T
Plasmid#24579DepositorInsertOfd1 (Ofd1 Mouse)
UseTagsMyc and eGFPExpressionMammalianMutationAlanine 80 changed to threonine (codon GCA to ACA)PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
M71->M72-IRES-tauGFP LNL TV
Plasmid#15641DepositorInsertM71->M72-IRES-tauGFP LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
MOR23Hybrid->M71-IRES-tauGFP LNL TV
Plasmid#15622DepositorInsertMOR23Hybrid->M71-IRES-tauGFP LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-G139S
Plasmid#24581DepositorInsertOfd1 (Ofd1 Mouse)
UseTagsMyc and eGFPExpressionMammalianMutationGlycine 139 changed to serine (codon GGT to AGT)PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only