We narrowed to 14,399 results for: NTS

1. pYO734

   Plasmid

   #235753

   Purpose

   Protein expression of ScVPS30 and ZZ-ScVPS38

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Inserts

   VPS30 (VPS30 Budding Yeast)
   VPS38 (VPS38 Budding Yeast)
   

   Use

   Tags

   ZZ-3xTEV

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYO934

   Plasmid

   #235766

   Purpose

   Expression of ScVPS34 delta CBR1 L31-D55-3xFLAG undeer its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS34 delta CBR1
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   aa 31-55 deleted

   Promoter

   ScVPS34 promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYO760

   Plasmid

   #235759

   Purpose

   Expression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS38 delta C2
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   aa 1-154 deleted

   Promoter

   ScVPS38 promoter

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYO761

   Plasmid

   #235760

   Purpose

   Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VSP38 delta BARA2
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   aa 320-440 deleted

   Promoter

   ScVPS38 promoter

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYO765

   Plasmid

   #235762

   Purpose

   Expression of ScVPS38 delta C2 (1-154) -EGFP under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS38
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   aa 1-154 deleted

   Promoter

   ScVPS38 promoter

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYO766

   Plasmid

   #235763

   Purpose

   Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VSP38 delta BARA2
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   aa 320-440 deleted

   Promoter

   ScVPS38 promoter

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYO933

   Plasmid

   #235765

   Purpose

   Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VPS34 delta C154-V202
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   aa 154-202 deleted

   Promoter

   ScVPS34 promoter

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-PGUS

   Plasmid

   #207827

   Purpose

   FLAG-tagged PGUS driven by CMV promoter

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   PGUS
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK617

   Plasmid

   #179297

   Purpose

   Expresses dCas9 fused to VP64

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-HA-shift-IRES-GFP(KaganE21)

    Plasmid

    #52004

    Purpose

    Expresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-Shift
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A 2 nucleotide insertion was introduced between t…

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pAF0687 Pa01_attP-mCherry donor

    Plasmid

    #193466

    Purpose

    Promoterless mcherry donor for Pa01

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX nsp1 CoV2

    Plasmid

    #175512

    Purpose

    For protein expression of WT nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNTI647 dCas9-Mxi1 TetR KanMX

    Plasmid

    #139474

    Purpose

    Integrates CRISPRi effector and tet-responsive regulator

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Inserts

    dCas9-Mxi1
    Tet Repressor
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1 and pTEF1

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNTI825

    Plasmid

    #216633

    Purpose

    Cal-ID (V5) for AAV production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAF0689 Ef1a-Pa01_attB-BFP acceptor

    Plasmid

    #193472

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYO1125

    Plasmid

    #235742

    Purpose

    Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    p40 PX domain (NCF4 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    aa 2-149 with Cys at 2

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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