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SQT1665

Plasmid

#78744

Purpose

Human expression plasmid for LbCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pNP1954

Plasmid

#222730

Purpose

pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pNP568

Plasmid

#222725

Purpose

pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNP2157

Plasmid

#222732

Purpose

pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Helper (WT)

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI935

Plasmid

#216638

Purpose

cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI825

Plasmid

#216633

Purpose

Cal-ID (V5) for AAV production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

AAV

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNP1047

Plasmid

#222726

Purpose

pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI934

Plasmid

#216637

Purpose

Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHis APEX2-eIF4A1

Plasmid

#129645

Purpose

Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

eukaryotic translation initiation factor 4A1 (EIF4A1 Human)

Tags

6xHis and APEX2

Expression

Bacterial

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pNTI821

Plasmid

#216630

Purpose

Tet-inducible Cal-ID (V5) for Sleeping Beauty transposon system

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

Sleeping beauty transposon system

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pNTI822

Plasmid

#216631

Purpose

Tet-inducible cpTurboID (V5) for Sleeping Beauty transposon system

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

Sleeping beauty transposon system

Tags

V5

Expression

Mammalian

Mutation

L120P

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI823

Plasmid

#216632

Purpose

Cal-ID variant with T195/G196 split site, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID (split site variant)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUC Ad VAI

Plasmid

#175518

Purpose

For mammalian expression of adenovirus VAI RNA

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

Ad VAI RNA

Use

Adenoviral

Promoter

lac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPRai

Plasmid

#189938

Purpose

Expresses CRISPRai cassette under EF1a promoter

Depositor

Yu-Chen Hu

Article

Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

Insert

CRISPRai cassette

Expression

Mammalian

Promoter

CMV enhancer-rat EF1 alpha

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-ZNF746

Plasmid

#207825

Purpose

Dox-inducible expression of ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 (ZNF746 Human)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pNTI826

Plasmid

#216634

Purpose

cpTurboID (V5) for AAV production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

AAV

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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