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pMS26: pTns(PmcCAST)_ΔTniQ

Plasmid

#168159

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS27: pTns(PmcCAST)_ΔTnsD

Plasmid

#168160

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS25: pTns(PmcCAST)

Plasmid

#168158

Purpose

Inducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS7: pCascade(AvCAST)_entry

Plasmid

#168140

Purpose

Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Expression

Insect

Mutation

P418S

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm4_A

Plasmid

#145883

Purpose

Insect Expression of DmLSm4

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm4 (LSm-4 Fly)

Tags

V5-6xHis

Expression

Insect

Mutation

two silent mutations compared to the sequence of …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBEST-HipO

Plasmid

#114599

Purpose

Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

HipO

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 Halo RagB

Plasmid

#112753

Purpose

express Halo-RagB

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Halo-RagB (RRAGB Human)

Tags

Halo

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHS1625

Plasmid

#239834

Purpose

OrufIscB-REC E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1505

Plasmid

#239830

Purpose

OrufIscB-REC mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHS2096

Plasmid

#239835

Purpose

NovaIscB E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Insertion of REC domain from NbaCas9-1 and swap o…

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[∆ChEx]

Plasmid

#221199

Purpose

ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa142-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM-601[insert(+1) TA-rich]

Plasmid

#221196

Purpose

601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

601[insert(+1)TA-rich]

Use

Unspecified

Mutation

601 has an additional mutation added 22 bp from d…

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[R126A/R130A]

Plasmid

#221197

Purpose

ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

R126A, R130A

Promoter

T7

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHelz_1-1212_AH

Plasmid

#148882

Purpose

Insect Expression of DmHelz_1-1212

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz_1-1212 (CG9425 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHelz_1-1212_AH

Plasmid

#148886

Purpose

Insect Expression of DmHelz_1-1212

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz_1-1212 (CG9425 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6Biso1_1379-1723del1535-1624_P

Plasmid

#147204

Purpose

Bacterial Expression of HsTNRC6Biso1_1379-1723del1535-1624

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1379-1723del1535-1624 (TNRC6B Human)

Expression

Bacterial

Mutation

one non silent mutation T1486A compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6Biso1 1-1218_L

Plasmid

#146887

Purpose

Mammalian Expression of HsTNRC6Biso1_1-1218

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1-1218 (TNRC6B Human)

Expression

Mammalian

Mutation

4 non silent mutations, S441G, H1088R, Q1106R, Q1…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6Biso1_1218-1723_L

Plasmid

#146888

Purpose

Mammalian Expression of HsTNRC6Biso1_1218-1723

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1218-1723 (TNRC6B Human)

Expression

Mammalian

Mutation

one non silent mutation T1486A and a deletion of …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsXRN1_1535-1706_K

Plasmid

#146782

Purpose

Mammalian Expression of HsXRN1_1535-1706

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1_1535-1706 (XRN1 Human)

Expression

Mammalian

Mutation

one non silent mutation S1688P compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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