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pKM673

Plasmid

#134222

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 510-end

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b-M3C

Plasmid

#107087

Purpose

M. sedula PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T-MsePCNA2

Plasmid

#107064

Purpose

Expresses M. sedula PCNA2 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T-MsePCNA1

Plasmid

#107063

Purpose

Expresses M. sedula PCNA1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-S3C

Plasmid

#107090

Purpose

S. solfataricus PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-S1C

Plasmid

#107088

Purpose

S. solfataricus PCNA1 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-YS3

Plasmid

#107084

Purpose

S. solfataricus PCNA3 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-YS1

Plasmid

#107082

Purpose

S. solfataricus PCNA1 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-M2C

Plasmid

#107086

Purpose

M. sedula PCNA2 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-M1C

Plasmid

#107085

Purpose

M. sedula PCNA1 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-YM3

Plasmid

#107081

Purpose

M. sedula PCNA3 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-YM2

Plasmid

#107080

Purpose

M. sedula PCNA2 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-YM1

Plasmid

#107079

Purpose

M. sedula PCNA1 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T-MsePCNA3

Plasmid

#107065

Purpose

Expresses M. sedula PCNA3 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663K

Plasmid

#92239

Purpose

Express R663K mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663K mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Lysine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-W695del

Plasmid

#92241

Purpose

Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Tryptophan 695 deleted

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663Q

Plasmid

#92240

Purpose

Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663Q mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Glutamine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698A

Plasmid

#92237

Purpose

Express H698A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663A

Plasmid

#92238

Purpose

Express R663A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3C01

Plasmid

#70944

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc1 (Orc1 Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only