We narrowed to 733 results for: pEGFP-N

1. CIBN-rTetR

   Plasmid

   #183919

   Purpose

   Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   CIBN-rTetR (CIB1 Synthetic, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. nls-NgAgo-GK

   Plasmid

   #78253

   Purpose

   Mammalian expression of NgAgo (Argonaute from N. gregoryi).

   Depositor

   Chunyu Han

   Article

   Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   NgAgo
   

   Use

   Tags

   a nuclear localization signal (NLS)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-gamma2s-EGFP

   Plasmid

   #118040

   Purpose

   GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG2 (Gabrg2 Rat)
   

   Use

   Tags

   EGFP and cMyc epitope EQKLISEEDL inserted between…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAN008

   Plasmid

   #220049

   Purpose

   Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope (positive control).

   Depositor

   Blake Wiedenheft

   Article

   Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

   Insert

   flag-HA-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EB3-GFP

   Plasmid

   #190164

   Purpose

   Expresses EB3-GFP in mammalian cells

   Depositor

   Anna Akhmanova

   Article

   Stepanova et al J Neurosci. 2003 Apr 1;23(7):2655-64.

   Insert

   EB3-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-GalT

   Plasmid

   #11929

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Promoter

   Available Since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAV1-deltaN-mEGFP

   Plasmid

   #27707

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1, N-terminal truncated (60-178) (CAV1 Canis lupus familiaris)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   N-terminal truncation with AA 1-59 missing. The r…

   Promoter

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. ChR2-2A-Halo

   Plasmid

   #22047

   Purpose

   Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

   Depositor

   Edward Boyden

   Article

   Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

   Insert

   ChR2-GFP-2A-Halo-YFP
   

   Use

   Tags

   GFP and YFP

   Expression

   Mammalian

   Mutation

   Note that there is a S147R mut in YFP. This mutat…

   Promoter

   CMV

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-vox-EGFP

   Plasmid

   #79970

   Purpose

   mammalian GFP reporter plasmid, target site for Vika recombinase

   Depositor

   Frank Buchholz

   Article

   Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

   Insert

   2 vox-sites flanking neo in frame with EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-dCas9-HA-NLS--GFP-p300

    Plasmid

    #183925

    Purpose

    p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP-p300
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. HSF1-GFPN3

    Plasmid

    #32538

    Depositor

    Stuart Calderwood

    Article

    Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    CMV

    Available Since

    Oct. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. NLS-dCas9-HA-NLS-GFP-VP16

    Plasmid

    #183923

    Purpose

    VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP-VP16
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-GCaMP3bright

    Plasmid

    #74423

    Purpose

    Express fluorescent calcium indicator GCaMP3bright in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3bright
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D392A

    Promoter

    CMV

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-GCaMP3fast

    Plasmid

    #74422

    Purpose

    Express fluorescent calcium indicator GCaMP3fast in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3fast
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W4Y D356A

    Promoter

    CMV

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PCDHGC5-EGFP

    Plasmid

    #119933

    Purpose

    Protocadherin gamma C5 expression (EGFP-tag at the C-terminus).

    Depositor

    Angel de Blas

    Article

    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

    Insert

    PCDHGC5 (Pcdhgc5 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKIF1C_R169W-GFP

    Plasmid

    #185981

    Purpose

    Mammalian expression of human KIF1C with R169W mutation and GFP tag.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    R169W - mutation causing HSP (SPG58)

    Promoter

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKIF1C_P176L-GFP

    Plasmid

    #185980

    Purpose

    Mammalian expression of human KIF1C with P176L mutation and GFP tag.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    P176L - mutation causing HSP (SPG58)

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NLS-dCas9-HA-NLS-GFP4-VPR

    Plasmid

    #183924

    Purpose

    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP4-VPR
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. cmamxA1-GFP

    Plasmid

    #107195

    Purpose

    Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A1
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCh-Miro1(∆TM)-SspB

    Plasmid

    #194007

    Purpose

    Optogenetic tool

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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