We narrowed to 11,217 results for: Kars

1. CSII-IDR2-owlTRIMCyp-FOS K283D Q287D

   Plasmid

   #79068

   Purpose

   Express owl monkey TRIMCyp K283D, Q287D in mammalan cells

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   TRIM5Cyp (owl monkey) (K283D, Q287D)-FOS
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Mammalian

   Mutation

   Changed Lys 283 to Asp and Gln 287 to Asp

   Promoter

   CMV

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIG1_UL136_26kDa_Myc

   Plasmid

   #74955

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)

   Expression

   Mammalian

   Mutation

   delta (1-62) M78A M80A M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIG1_UL136_33kda_Myc

   Plasmid

   #74957

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Myc tag (EQKLISEEDL)

   Expression

   Mammalian

   Mutation

   M63A M78A M80A M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

   Plasmid

   #42185

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   G protein Gamma 3(F70A, F71A) (Gng3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   F70A, F71A

   Promoter

   cmv

   Available since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1

   Plasmid

   #42188

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   Gamma 3(K68A,K69A) (Gng3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K68A,K69A

   Promoter

   cmv

   Available since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1(+) NAPstar3b

   Plasmid

   #232736

   Purpose

   Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.

   Depositor

   Bruce Morgan

   Article

   Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

   Insert

   NAPstar3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61591

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAR17_StrepI_hFASh_H8_pET22b

   Plasmid

   #122846

   Purpose

   Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   human FASN/ human fatty acid synthase (FASN Human)
   

   Use

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAR18_StrepI_mFASm_H8_pET22b

   Plasmid

   #122847

   Purpose

   Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
   

   Use

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE7

    Plasmid

    #214812

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-MS2V5-eIF4G delN83

    Plasmid

    #65812

    Purpose

    expression of eIF4G-MS2 fusion.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eIF4G (EIF4G1 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    delN83 (functionally behaves as the WT protein)

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid

    #128604

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CLP1

    Plasmid

    #155462

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CLP1 (CLP1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-UbcP-Myc-YWAA-pGK-HYG

    Plasmid

    #107377

    Purpose

    Lentiviral expression of myc-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO_FOXA1_#1

    Plasmid

    #70095

    Purpose

    Expression of shRNA to human FOXA1, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO_FOXA1_#2

    Plasmid

    #70096

    Purpose

    Expression of shRNA to human FOXA1, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7_CC_PE7_IVT_Template

    Plasmid

    #223022

    Purpose

    Template for in vitro transcription of PE7. For HSPC-related experiments.

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DDX5

    Plasmid

    #155523

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-PE7 for IVT

    Plasmid

    #214813

    Purpose

    Template for in vitro transcription of PE7

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJT305 Pa01 Lenti pEF-attB LSR GFP

    Plasmid

    #184948

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Michael Bassik, Ami Bhatt, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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