We narrowed to 5,842 results for: pcas

1. pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

   Plasmid

   #218870

   Purpose

   Mammalian expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2(no bind)
   

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   CAG

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GB3668

   Plasmid

   #215243

   Purpose

   TU for the expression of mAID (N-tag): AcrIIA4 w/o NLS protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and TNOS terminator

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   P35S:mAID:AcrIIA4:TNOS
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GB1531

   Plasmid

   #215242

   Purpose

   TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   PNos:PhiC31:TNos
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCPH3.1-GS3

   Plasmid

   #204762

   Purpose

   Expression of Cas9 and human H3.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H3.1 (H3C6 Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCPH2A-GS3

   Plasmid

   #204753

   Purpose

   Expression of Cas9 and human H2A

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2A (H2AC20 Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCPH2A.1-GS3

   Plasmid

   #204754

   Purpose

   Expression of Cas9 and human H2A.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2A.1 (H2AC17 Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCPH2A.2-GS3

   Plasmid

   #204755

   Purpose

   Expression of Cas9 and human H2A.2

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2A.2 (H2AC18 Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCPH2A.J-GS3

   Plasmid

   #204757

   Purpose

   Expression of Cas9 and human H2A.J

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AJ (H2AJ Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psgRNA-GST-EGFP-GPIBY55

   Plasmid

   #213720

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Promoter

   CMV

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psgRNA-GST-EGFP-GPIDAF

    Plasmid

    #213719

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psgRNA-GST-EGFP-GPICEAM7

    Plasmid

    #213721

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCLV3Cas-LT2

    Plasmid

    #210550

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
    

    Expression

    Plant

    Mutation

    1bp insertion after AAVS1 target sequence that di…

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ABE-RA5.5

    Plasmid

    #208316

    Purpose

    Expresses ABE-RA5.5 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA5.5-SpCas9 D10A
    

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hPRL2_1-163

    Plasmid

    #209794

    Purpose

    Bacterial expression of human PRL-2 without farnesylation motif

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

    Insert

    PRL-2 (PTP4A2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 1-163 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pROF018

    Plasmid

    #155325

    Purpose

    Constitutive expression of KRAB-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-NLS-KRAB-EL222-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pROF050

    Plasmid

    #155327

    Purpose

    Constitutive expression of BRD-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-BRD-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HSF2 KO

    Plasmid

    #200208

    Purpose

    gRNA for HSF2 KO

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HSF2 KO (Hsf2 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TBPL1 KO 1

    Plasmid

    #200210

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 1 (Tbpl1 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TBPL1 KO 2

    Plasmid

    #200211

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 2 (Tbpl1 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197958

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    CmYLCVp and pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge