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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 5021 - 5040 of 12282 results

  1. pGBW-m4133908

    Plasmid
    #152258
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152258-plasmid-map-sequence-id-300228

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  2. pGBW-m4046299

    Plasmid
    #153878
    Purpose
    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153878-plasmid-map-sequence-id-300422

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  3. pGBW-m4046179

    Plasmid
    #149059
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149059-plasmid-map-sequence-id-297954

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  4. pGBW-m4046451

    Plasmid
    #145567
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145567-plasmid-map-sequence-id-332021

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  5. pGBW-m4046457

    Plasmid
    #145424
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145424-plasmid-map-sequence-id-298353

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  7. pYPQ239-RR

    Plasmid
    #108862
    Purpose
    FnCpf1 Gateway entry plasmid with N623R and K687R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N623R and K687R, Cpf1 is rice codon optimized
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108862-plasmid-map-sequence-id-274177

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  10. 4QPP

    Plasmid
    #61964
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)
    Use
    Baculovirus expression
    Tags
    6x His and TEV cleavage site
    Mutation
    See comments
    Promoter
    Polyhedrin
    Available since
    Sept. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pCAG-HA-NLGN4 D396*

    Plasmid
    #59321
    Purpose
    Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation
    Depositor
    Peter Scheiffele
    Publication
    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLGN4 D396* (NLGN4X Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D396* nonsense mutation, rest of ORF deleted
    Promoter
    CAG
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    59321-plasmid-map-sequence-id-107090

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  12. PREX1

    Plasmid
    #32041
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PREX1 (PREX1 )
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    first PDZ domain only (amino acids Residues 607-7…
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pCDH-EF1-E-'No'Mi-Red-T2A-Puro

    Plasmid
    #246642
    Purpose
    EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.
    Depositor
    Koen Breyne
    Publication
    Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70
    Insert
    cleavable E-NoMi-Red
    Use
    Lentiviral
    Tags
    FLAG-Nanoluciferase and mCherry
    Expression
    Mammalian
    Promoter
    EF-1alpha
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246642-plasmid-map-sequence-id-489517

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  16. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid
    #208043
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO2nc (SUMO2 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q90P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208043-plasmid-map-sequence-id-410267

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  19. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  20. pCjedCas9

    Plasmid
    #172216
    Purpose
    Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells
    Depositor
    John van der Oost
    Publication
    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    Tags
    Histag
    Mutation
    changed Asp 8 to Ala and His 559 to Ala
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    172216-plasmid-map-sequence-id-338133

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Showing: 5021 - 5040 of 12282 results