We narrowed to 15,981 results for: LEA

1. SuExp-mPLD3

   Plasmid

   #201245

   Purpose

   express full length mouse PLD3 protein

   Depositor

   David Nemazee

   Article

   Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

   Insert

   PLD3 (Pld3 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIPZ-Myr-PTEN

   Plasmid

   #206983

   Purpose

   Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

   Depositor

   Ming Chen

   Article

   Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   N-terminal myristoylation site

   Expression

   Mammalian

   Promoter

   tet-on promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOCC292

   Plasmid

   #118881

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
   

   Tags

   mRuby3, HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEn-C1.1

   Plasmid

   #61479

   Purpose

   Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

   Depositor

   Holger Puchta

   Article

   Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   U6-26

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1V5-His Snk/Plk2 T239D

   Plasmid

   #22220

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, threonine …

   Available Since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

6. MSCV-mNINJ1-D53A-GFP

   Plasmid

   #208778

   Purpose

   MSCV vector expressing D53A mouse NINJ1

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   NINJ1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Mutation

   Changed aspartic acid 53 to alanine (D53A)

   Available Since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET41a iGluSnFR

   Plasmid

   #119828

   Purpose

   Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

   Plasmid

   #197968

   Purpose

   Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   C328S, C331S, L1332V

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-11

   Plasmid

   #172762

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-11
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+ macroH2A1.2-GFP-HA

    Plasmid

    #30515

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    macroH2A1.2-C (Macroh2a1 Mouse)
    

    Use

    Xenopus/zebrafish expression.

    Tags

    eGFP and triple HA

    Expression

    Mammalian

    Mutation

    NO STOP

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTSSX-STC

    Plasmid

    #115668

    Purpose

    E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

    Depositor

    David Kramer

    Article

    Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

    Insert

    small tetraheme cytochrome c
    

    Tags

    Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC291

    Plasmid

    #118867

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mRuby3

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pClneoMyc human MOB1

    Plasmid

    #37024

    Depositor

    Yutaka Hata

    Article

    Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

    Insert

    MOB1B (MOB1B Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pET21-pelB-LaG-21

    Plasmid

    #172749

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-21
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-37

    Plasmid

    #172756

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-37
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDARMO_CMVT_N_Strep_BIRC6

    Plasmid

    #197972

    Purpose

    Mammalian cell expression of wt Strep-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    L1332V

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pet15b-Endosomal Escape Control

    Plasmid

    #186552

    Purpose

    Negative Control confirming the need for a CPP peptide in mediating internalization

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    6H-ETA-GFP
    

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pet15b-Internalization and Endosomal Escape

    Plasmid

    #186551

    Purpose

    Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    10R-8His-ETA-EGFP
    

    Tags

    8xHis and EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HS-OS-Plk4-mKate2

    Plasmid

    #105944

    Purpose

    heat shock inducible transgenesis, non phoshorylable version of Plk4

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Plk4 (Plk4 Mouse)
    

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    1002 to 3704 of Plk4/ deletion of S282-S305.

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. cycLuc_PPVs

    Plasmid

    #119208

    Purpose

    Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with PPVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Promoter

    CMV

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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