We narrowed to 40,077 results for: KAN;

1. pJRH-1179 U6-reci Gag-Cas9 v2

   Plasmid

   #201914

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-Cas9 v2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti EF1a GFP-mCherry-mPlin1

   Plasmid

   #189005

   Purpose

   Fluorescent reportor for lipophagy

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   GFP, mChery, Plin1 (Plin1 Mouse)
   

   Use

   Lentiviral

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA3790

   Plasmid

   #70110

   Purpose

   mtDNA elimination/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   hUNG1 Y147A
   Neo
   

   Tags

   None, human OTC mitochondrial targeting sequence,…

   Expression

   Mammalian

   Mutation

   Y147A, aa's 1-77 removed

   Promoter

   CMV, EF1a, and SV40 and native

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH-1180 U6-reci Gag-pol v2

   Plasmid

   #201915

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-pol
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMIIS793

   Plasmid

   #238206

   Purpose

   Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Tags

   TwinStrepII-bdSUMO

   Expression

   Bacterial

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER21-puro_Gateway-3xFLAG

   Plasmid

   #172981

   Purpose

   Gateway Destination vector for doxycycline-inducible expression of ORF

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSD080A

   Plasmid

   #218138

   Purpose

   Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD40-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Expression

   Mammalian

   Promoter

   CAG and Human U6

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGM65881

   Plasmid

   #153215

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. slc1a3b:myrGFP-P2A-H2AmCherry

    Plasmid

    #170208

    Purpose

    Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

    Depositor

    Kelly Monk

    Article

    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

    Insert

    slc1a3b (slc1a3b Zebrafish)
    

    Use

    Zebrafish

    Tags

    myrGFP-P2A-H2AmCherry

    Promoter

    slc1a3b

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5_mCherry_P2AT2A_GFP-nb

    Plasmid

    #238232

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. HypoxCR

    Plasmid

    #59946

    Purpose

    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

    Depositor

    Anne Le

    Article

    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

    Inserts

    GFP-Pest
    mCherry-Geminin
    

    Use

    Lentiviral

    Tags

    FLAG and PEST

    Expression

    Mammalian

    Promoter

    CMV and min CMV

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. mActA-FKBP-mCherry

    Plasmid

    #210295

    Purpose

    ActuAtor for FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    mActA-FKBP-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPUR-mU6-sgRNA-2XPP7

    Plasmid

    #194504

    Purpose

    Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

    Plasmid

    #170209

    Purpose

    Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

    Depositor

    Kelly Monk

    Article

    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

    Insert

    slc1a3b (slc1a3b Zebrafish)
    

    Use

    Zebrafish

    Tags

    myrGCaMP6s-P2A-H2AmCherry

    Promoter

    slc1a3b

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoG

    Plasmid

    #238235

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS-FtoG
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5_mCherry-P300

    Plasmid

    #238258

    Purpose

    For overexpression of mCherry-P300

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P300
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-G-GECO1.0

    Plasmid

    #32447

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.0
    

    Expression

    Mammalian

    Mutation

    GCaMP3 K119I/L173Q/S404G/E430V

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRE-KRAB-dCas9-IRES-GFP

    Plasmid

    #85556

    Purpose

    2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB_TetON_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238240

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Expression

    Mammalian

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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