We narrowed to 14,429 results for: nts

1. pJBL712

   Plasmid

   #140382

   Purpose

   Expresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-otrO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTX201

   Plasmid

   #89265

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA02

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsYSA-gRNA02
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOD_A3601

   Plasmid

   #91051

   Purpose

   Module A, Promoter: 35S, Gene: dAtCas9-VP64, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   dAtCas9-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   35S

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T1 human APC-2,3 (1362–1540)

   Plasmid

   #132631

   Purpose

   expresses human APC-2,3 (1362–1540) in E. coli

   Depositor

   Wenqing Xu

   Article

   Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

   Insert

   APC-2,3 (1362–1540)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH71

   Plasmid

   #128214

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5_tAKARζ

   Plasmid

   #119918

   Purpose

   Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARζ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUPI AoT5Cyp

   Plasmid

   #80280

   Purpose

   Expresses Aotus TRIM5 CypA fusion

   Depositor

   Jeremy Luban

   Article

   Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

   Insert

   TRIM5 CypA fusion
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR2KN0079

   Plasmid

   #117646

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-1459-adcy4-Luc

   Plasmid

   #28184

   Depositor

   Bernard Schimmer

   Article

   Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

   Insert

   Adcy4 promoter (Adcy4 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2011

   Availability

   Academic Institutions and Nonprofits only

10. AAV-oChD-citrine

    Plasmid

    #50976

    Purpose

    AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    oChD-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChD gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. CP1300-CBEmax

    Plasmid

    #119805

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1300) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT mir-1-1 reporter cel-mir-40

    Plasmid

    #46739

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    cel-mir-40
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (MT)

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-SZT2 1-1021

    Plasmid

    #87037

    Purpose

    mammalian expression of SZT2 fragment

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 1-1021

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPOR0SP0016

    Plasmid

    #117526

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K810A, K1003A, R1060A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS ZfHoxd3a

    Plasmid

    #27534

    Depositor

    Victoria Prince

    Article

    Prince et al Development. 1998 Feb . 125(3):393-406.

    Insert

    Hoxd3a (hoxd3a Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pDIRECT_37J

    Plasmid

    #91153

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:TALEN
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PI(DM)-Rac1(WT)dc1.FLARE

    Plasmid

    #87589

    Purpose

    PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    PBD
    Rac1
    

    Use

    Tags

    YPet and dTurq

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHJ32

    Plasmid

    #48361

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD_A1310

    Plasmid

    #91034

    Purpose

    Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_H840A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    H840A

    Promoter

    ZmUbi

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

    Plasmid

    #68274

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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