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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,107 results for: transfer

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Showing: 5041 - 5060 of 10107 results

  4. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  8. pNUTN6His Y426F/Y517F hTFNG

    Plasmid
    #70084
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70084-plasmid-map-sequence-id-129389

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  10. pcDNA3-ΔN-RNMT

    Plasmid
    #112708
    Purpose
    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10
    Insert
    RNMT (RNMT Human)
    Tags
    FLAG and HIV Rev nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-120, mutated active-site D2…
    Promoter
    CMV
    Available Since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112708-plasmid-map-sequence-id-217040

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  11. pNUT N6His K206E hTFNG

    Plasmid
    #70130
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    He et al Biochemistry. 1999 Jul 27;38(30):9704-11
    Insert
    mutated human serum transferrin (TF Human)
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid, Asn413Asp, Asn…
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70130-plasmid-map-sequence-id-125031

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  13. pNUT N6His Y95F/Y188F hTFNG

    Plasmid
    #69601
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    69601-plasmid-map-sequence-id-124729

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  20. pLenti PGK mKate2-OGT K842M (Puro)

    Plasmid
    #154291
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    K842M mutation (Catalytic Inactive)
    Promoter
    PGK
    Available Since
    Dec. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154291-plasmid-map-sequence-id-332432

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Showing: 5041 - 5060 of 10107 results