168,871 results

1. Red Glifon 300

   Plasmid

   #163115

   Purpose

   Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

   Depositor

   Takashi Tsuboi

   Article

   Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

   Insert

   Red Glifon 300
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJYS1Ptac

   Plasmid

   #85545

   Purpose

   Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Expression

   Bacterial

   Promoter

   tac and unknown

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2-RB1

   Plasmid

   #87836

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Insert

   sgRB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Expression

   Mammalian

   Promoter

   Tight TRE promoter and U6

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFPGUSPlus

   Plasmid

   #64401

   Purpose

   binary plant vector expressing GFP and GUS, both driven by a 35S promoter

   Depositor

   Claudia Vickers

   Article

   Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

   Inserts

   GFP
   GUSPlus
   

   Use

   Binary vector for plant transformation

   Tags

   His

   Expression

   Plant

   Mutation

   S65T

   Promoter

   35S

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbE8k-RFP

   Plasmid

   #35270

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BAD

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

   Plasmid

   #85756

   Purpose

   All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Inserts

   Csy4-T2A-SpRFN-P2A-GFP
   gRNA
   

   Use

   CRISPR

   Tags

   Csy4, Csy4 recognition sequence, FokI fusion via …

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CMV and U6

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB405

   Plasmid

   #74799

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH-nCas9-PPE-V2

   Plasmid

   #170131

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

   Insert

   nCas9(H840A)-M-MLV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   H840A for Cas9; D200N, T306K, W313F, T330P and L…

   Promoter

   maize Ubiquitin-1, OsU3

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2815

   Plasmid

   #144244

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOSB (FOSB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXB-EBNA1

    Plasmid

    #41857

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3TS-RfxCas13d-HA

    Plasmid

    #141320

    Purpose

    Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    HA

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGK-HELLO LV

    Plasmid

    #202001

    Purpose

    Expresses HELLO neoantigen in tumor cells

    Depositor

    Nikhil Joshi

    Article

    Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

    Insert

    HELLO
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    pGK

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDA_867

    Plasmid

    #231109

    Purpose

    A>G base editor

    Depositor

    John Doench, David Root

    Article

    Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

    Insert

    ABE8e-nCas9-SpG-HA
    

    Use

    CRISPR and Lentiviral

    Mutation

    D10A

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-mStayGold-EFS-Blast

    Plasmid

    #227257

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CaV2.1 (pcDNA6)

    Plasmid

    #26578

    Depositor

    Diane Lipscombe

    Article

    Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

    Insert

    Cacna1a (Cacna1a Mouse)
    

    Tags

    His6 and V5

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV.TBG.PI.Cre.rBG

    Plasmid

    #107787

    Purpose

    AAV vector expressing Cre from TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TBG

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188766

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS088

    Plasmid

    #211502

    Purpose

    AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. xlox(GFP)hTERT

    Plasmid

    #69809

    Purpose

    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a GFP marker

    Depositor

    David Ott

    Insert

    hTERT Reverse Transcriptase (TERT )
    

    Use

    Retroviral

    Tags

    none

    Promoter

    MLV promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTKLP-tetA

    Plasmid

    #71325

    Purpose

    tetA landing pad template plasmid

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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