We narrowed to 152,916 results for: DES

1. pBEG R2-dsRed-L3 #BGV203

   Plasmid

   #48973

   Purpose

   Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   dsRed
   

   Use

   Gateway cloning vector

   Tags

   IRES (strong)

   Expression

   Mutation

   Promoter

   none

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pBEG R2-iBlast-L3 #BGV204

   Plasmid

   #48982

   Purpose

   Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   blasticidin-S deaminase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Expression

   Mutation

   Promoter

   na

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLEG(R1-R4) #BGV057

   Plasmid

   #48957

   Purpose

   Lentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pMXs_NMNAT3-FLAG

   Plasmid

   #133261

   Purpose

   Express FLAG-tagged NMNAT3 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NMNAT3 (NMNAT3 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEG R3-miRNA(ccdB)-L4 #BGV228

   Plasmid

   #48953

   Purpose

   To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   na

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pBEG R2-iHygro-L3 #BGV206

   Plasmid

   #48985

   Purpose

   Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   hygromycin phosphotransferase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Expression

   Mutation

   Promoter

   na

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. FUW-cFos

   Plasmid

   #193089

   Purpose

   constitutive expression of mouse cFos in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   cFos
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR223-BALF5

   Plasmid

   #198577

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BALF5 (BALF5 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   None (wildtype)

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR223-BALF2

   Plasmid

   #198578

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BALF2 (BALF2 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   None (wildtype)

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABE-SSeCKs T751A

    Plasmid

    #107142

    Purpose

    full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

    Depositor

    Heidi Hehnly

    Article

    Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

    Insert

    SSeCKs T751A (Akap12 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed threonine 751 to alanine

    Promoter

    gag

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSUMO His6 SUMO AMPKa (11-281)

    Plasmid

    #177851

    Purpose

    Bacterial Expression of AMPKa1

    Depositor

    Karsten Melcher

    Article

    Li et al Cell Res. 2015 Jan;25(1):50-66. doi: 10.

    Insert

    AMPK a1 (PRKAA1 Human)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti hSyn PGK1-HALO

    Plasmid

    #220910

    Purpose

    Neuronal specific expression of the fusion construct PGK1-HALO

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-HALO (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1_miRFP703

    Plasmid

    #183759

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    miRFP703
    

    Use

    Tags

    Lck lipidation signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLJM60 FLAG-RAB35 Q67L

    Plasmid

    #107105

    Purpose

    Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

    Depositor

    Charles Sawyers

    Article

    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PXPR007 sgGFP-1

    Plasmid

    #74960

    Purpose

    Cas9 + sgGFP-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmcherry α-catenin L344P

    Plasmid

    #178647

    Purpose

    express α-catenin with L344P mutation

    Depositor

    Daniel Conway

    Article

    Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

    Insert

    α-catenin (CTNNA1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    L344P

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK_ToeRepG2_N02_switch

    Plasmid

    #132742

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N02_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Str-KDEL_ST-SBP-tagBFP

    Plasmid

    #65266

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57mini ZM249M P90A _env eGFP

    Plasmid

    #132939

    Purpose

    pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    HIV-1ZM249M provirus (P90A) with env deletion, GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    P90A

    Promoter

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ii-Str_ST-SBP-mCherry

    Plasmid

    #65269

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge