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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PP165

Plasmid

#78985

Purpose

Supplemental Figure 4. Promoter GCW14. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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LI C-D 1-10GFP noATG

Plasmid

#54462

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

superfolder GFP 1-10 domain without start codon

Use

Cloning vector

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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PP161

Plasmid

#78993

Purpose

Supplemental Figure 4. Promoter GAP3. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP164

Plasmid

#78988

Purpose

Supplemental Figure 4. Promoter short ppTEF1. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP149

Plasmid

#78989

Purpose

Supplemental Figure 4. Promoter WT GAP. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP154

Plasmid

#78991

Purpose

Supplemental Figure 4. Promoter GAP1. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP160

Plasmid

#78992

Purpose

Supplemental Figure 4. Promoter GAP2. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP155

Plasmid

#78994

Purpose

Supplemental Figure 4. Promoter GAP4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP162

Plasmid

#78995

Purpose

Supplemental Figure 4. Promoter GAP5. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP163

Plasmid

#78996

Purpose

Supplemental Figure 4. Promoter GAP6. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Hsyn-Sun1sfGFP

Plasmid

#222510

Purpose

AAV packaging construct for expression Sun1-sfGFP fusion Protein under the human synapsin 1 gene promoter

Depositor

Sarkis Mazmanian

Insert

Sun1-sfGFP (Sun1 Mouse)

Use

AAV

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL3986

Plasmid

#204132

Purpose

Thermotoga petrophila (T. pe) fluoride riboswitch, wild type

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

T. pe fluoride riboswitch sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL811

Plasmid

#204135

Purpose

Low-copy constitutively expressed sfGFP for E. coli

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL830

Plasmid

#204136

Purpose

Thermotoga petrophila (T. pe) fluoride riboswitch, D4

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

T. pe fluoride riboswitch with decreasing mutant at position 4 regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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SMT000

Plasmid

#216849

Purpose

Plasmid that encodes a sfGFP gene under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramphenicol resistant.

Depositor

Polly Fordyce

Article

Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

Insert

Superfolder Green Fluorescent Protein

Use

Synthetic Biology

Expression

Bacterial

Mutation

S30R, Y39N, N105T, Y145F, I171V, and A206V

Promoter

T7

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-psfGFPcp-hphNT1

Plasmid

#240558

Purpose

Tag S. pombe genes at 3' end (C-terminal end of protein) with circularly permuted superfolder GFP, codon-optimized for S. pombe

Depositor

Silke Hauf

Article

Kamenz et al Mol Cell. 2015 Nov 5;60(3):446-59. doi:

Insert

psfGFPcp

Expression

Yeast

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB035

Plasmid

#239575

Purpose

Deactivated ribozyme split sfGFP expression

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Insert

Superfolder GFP with deactivated ribozyem insertion

Use

Synthetic Biology

Expression

Plant

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB034

Plasmid

#239574

Purpose

Ribozyme split sfGFP expression

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Insert

Superfolder GFP with ribozyem insertion

Use

Synthetic Biology

Expression

Plant

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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