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pBSV2G_PflaB-mCeruleanBb

Plasmid

#118235

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCerulean

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPBAD-yhjH

Plasmid

#111613

Purpose

Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

Depositor

Tim Tolker-Nielsen

Article

Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

Insert

yhjH

Expression

Bacterial

Mutation

A52V

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57-mCherry-2A-BRD4 Iso AdelCTD

Plasmid

#137722

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Truncated at amino acid 1328

Promoter

Tight TRE promoter

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJet1.2 sfGFP

Plasmid

#117123

Purpose

C-terminal GFP tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Superfolder GFP

Use

Golden gate donor vector for gene targeting in ba…

Tags

sfGFP

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCMVblast_SLC38A2_wt

Plasmid

#156180

Purpose

CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA se…

Promoter

CMV

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Epac1 SAAX

Plasmid

#118313

Purpose

Expression of human Epac1

Depositor

Philip Stork

Article

Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

Insert

Epac1 SAAX (RAPGEF3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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mTFP1-TOMM20-N-10

Plasmid

#55511

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

TOMM20 (TOMM20 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pRST11B-AS3_4

Plasmid

#102789

Purpose

Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Inserts

tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
suppressor tRNA

Expression

Bacterial

Available Since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pAV-U6+27-Tornado-Pepper-RhoBAST

Plasmid

#196343

Purpose

Overexpression of circular Pepper-RhoBAST

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

Tornado-Pepper-RhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti Vp64-dCas9 T2A mTagBFP2

Plasmid

#171052

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Vp64-dCas9 T2A mTagBFP2

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/Lux-nat

Plasmid

#120324

Purpose

Bacterial luminescence

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Expression

Bacterial

Promoter

proD

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Expression

Bacterial

Promoter

U6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pHLU

Plasmid

#64167

Purpose

harbours marker genes HIS3, LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx2(yeast)

Plasmid

#65005

Purpose

Yeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

GRX2

Tags

roGFP2

Expression

Yeast

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pAC-PHYT

Plasmid

#19002

Depositor

Jay Keasling

Article

Smolke et al Metab Eng. 2001 Oct . 3(4):313-21.

Insert

crtE, crtB

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pCMV3Tag8li-hDDX50-HA

Plasmid

#165465

Purpose

Human DDX50 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DExD-box helicase 50 (DDX50 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Plasmid

#159859

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-TGAT

Plasmid

#84334

Purpose

Expresses TGAT tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

TGAT (TRIO Human)

Tags

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I beta

Plasmid

#22155

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pU6_HEK3+1T>A_(PP7-C4-Q1)

Plasmid

#232437

Purpose

pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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