We narrowed to 13,156 results for: otos

1. iRFP713-N2-Frankenbody-Flag

   Plasmid

   #175291

   Purpose

   Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with iRFP713
   

   Use

   Tags

   iRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pS848∙CsR

   Plasmid

   #199240

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

   Plasmid

   #200475

   Purpose

   Lentiviral vector expressing gRNA targeting GFP

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

   Plasmid

   #202617

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   FRT-JEDI-1P-Kv
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_Flex_syn_SpikeyGi2_kv2.1

   Plasmid

   #195314

   Purpose

   Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

   Depositor

   Vincent Pieribone

   Article

   Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

   Insert

   SpikeyGi2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUB-nSypHer (NLS:sypHer:NLS)

   Plasmid

   #84731

   Purpose

   Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   nSypHer
   

   Use

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Mutation

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCerulean-LaminB1-10

   Plasmid

   #55380

   Purpose

   Localization: Nuclear Envelope, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

   Plasmid

   #159434

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Inserts

   Neomycin
   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNAIIIB HA JNK

    Plasmid

    #61630

    Purpose

    mammalian expression of JNK

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    JNK1 beta1 isoform (MAPK8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-mCherry-WPRE

    Plasmid

    #202620

    Purpose

    Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCerulean3-Golgi-7

    Plasmid

    #55420

    Purpose

    Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pECE-M2-PP1Cbeta

    Plasmid

    #31677

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

15. mTagRFP-T-Actin-7

    Plasmid

    #58001

    Purpose

    Localization: Actin, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Use

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

    Plasmid

    #99221

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCerulean3-Fibrillarin-7

    Plasmid

    #55419

    Purpose

    Localization: Nucleoli, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

19. CAG::Gdnf

    Plasmid

    #78209

    Purpose

    piggybac vector for mouse Gdnf expression

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    Gdnf (Gdnf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

20. mKO-Mito-7

    Plasmid

    #57848

    Purpose

    Localization: Mitochondria, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mKO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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