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pVP079

Plasmid

#222031

Purpose

MultiGreen 2.0 BC level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP080

Plasmid

#222032

Purpose

MultiGreen 2.0 CD level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP081

Plasmid

#222033

Purpose

MultiGreen 2.0 DE level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP082

Plasmid

#222034

Purpose

MultiGreen 2.0 EF level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP083

Plasmid

#222035

Purpose

MultiGreen 2.0 FG level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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insulator_seq_barcode_vector

Plasmid

#221458

Purpose

Vector for generating a barcode library for insulator-seq.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Type

Empty backbone

Use

Synthetic Biology and Unspecified

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

Plasmid

#196846

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

Plasmid

#196845

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMYT006

Plasmid

#203322

Purpose

Encodes pLacHTA1 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pLacHTA1

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1844-2361-HCAtoEEE_AD

Plasmid

#148485

Purpose

Bacterial Expression of HsNot1iso1_1844-2361-HCAtoEEE

Depositor

Elisa Izaurralde

Insert

HsNot1iso1_1844-2361-HCAtoEEE (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKP2304

Plasmid

#167633

Purpose

Express TonB C18G, Q160C, F202A, W213A.

Depositor

Kathleen Postle

Article

Postle et al bioRxiv 2021.12.08.471779

Insert

tonB C18G, Q160C, F202A, W213A

Expression

Bacterial

Mutation

tonB with C18G, Q160C, F202A, W213A mutations. Pr…

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF038

Plasmid

#157635

Purpose

Empty vector

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Type

Empty backbone

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNK083b

Plasmid

#156366

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S16 ribosomal gene

Use

Synthetic Biology

Available Since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 LEC2t

Plasmid

#153376

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

LEC2 terminator

Expression

Plant

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 APT1t

Plasmid

#153386

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

APT1 terminator

Expression

Plant

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 NOSp

Plasmid

#153375

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

NOS promoter

Expression

Plant

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 APT1p

Plasmid

#153372

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

APT1 promoter

Expression

Plant

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 35Sp

Plasmid

#153369

Purpose

Cauliflower mosaic virus promoter for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

35S promoter

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 ACT2t

Plasmid

#153384

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

ACT2 terminator

Expression

Plant

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0287

Plasmid

#154502

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MMV(ΔTCP3)

Use

Synthetic Biology

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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