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pGWB427

Plasmid

#74821

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB426

Plasmid

#74820

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPBpuro-mNG-eDHFR(69K6)-Tiam1

Plasmid

#172102

Purpose

Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB422

Plasmid

#74816

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB428

Plasmid

#74822

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMM549

Plasmid

#112531

Purpose

pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA

Expression

Bacterial

Promoter

J23107 and ProD

Available Since

Feb. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p53-(1-393)-E285A-N268D

Plasmid

#35090

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - E285A-N268D

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 T538N

Plasmid

#60537

Purpose

Donor vector for human Pak5 T538N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

T538N

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S573N

Plasmid

#60538

Purpose

Donor vector for human Pak5 S573N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

S573N

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pScalps_puro_mklf1

Plasmid

#59918

Purpose

Expresses mouse Kruppel-like factor1

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

Kruppel-like factor 1 (Klf1 Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
p53-(1-393)-E285V-N268D

Plasmid

#35088

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - E285V-N268D

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pPD95.75-cYAP-1

Plasmid

#66857

Purpose

Generate transgenic C.elegans with cYAP-1 fused to GFP

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

C.elegans YAP-1 (yap-1 Nematode)

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

C. elegans 5'UTR

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pPD95.75-cYAP-1 S104A

Plasmid

#66858

Purpose

Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pUC-FLAG-V2-REPAIR-mini

Plasmid

#252298

Purpose

Expresses REPAIR-mini (V2) in mammalian cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR-mini (V2)

Expression

Mammalian

Mutation

dPspCas13b H133A/H1058A/Δ929–1090; 30-aa linker; …

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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PACT wild type

Plasmid

#251697

Purpose

PRKRA protein expression construct in E. coli

Depositor

Sun Hur

Article

Ahmad et al Nat Commun. 2025 Apr 8;16(1):3325. doi:

Insert

PRKRA (PRKRA Human)

Expression

Bacterial

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pFastBac-V2-REPAIR-mini

Plasmid

#252297

Purpose

Expresses REPAIR-mini (V2) in insect cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR-mini (V2)

Expression

Insect

Mutation

dPspCas13b H133A/H1058A/Δ929–1090; 30-aa linker; …

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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TEVEp_pRK793

Plasmid

#247672

Purpose

pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVEp insert based on Yi et al., 2013.

Depositor

Carl Denard

Article

Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2

Insert

MBP-ENLYFES-7xHIS-TEVEp

Tags

His and MBP

Expression

Bacterial

Mutation

S120R, D148R, T173A, N177K, M218I, S219P

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-hSYN-MYC-MIRO1

Plasmid

#219577

Purpose

Lentiviral construct expressing MYC-tagged human Miro1

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Miro1 (RHOT1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Promoter

hSYN1

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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hTRAK1(S719A, S919A)

Plasmid

#225226

Purpose

Expresses MYC-tagged hTRAK1(S719A, S919A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

S719A, S919A mutations

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-hSYN-MYC-TRAK1

Plasmid

#228547

Purpose

MYC-tagged hTRAK1 expressed from the hSYN1 promoter.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Promoter

hSYN1

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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