We narrowed to 17,414 results for: LEA

1. pLSU4cg10

   Plasmid

   #126078

   Purpose

   Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   Chloroplastic FNR transit peptide
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Promoter

   longCaMV35S

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTG131

   Plasmid

   #198640

   Purpose

   Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   Cas9
   

   Expression

   Bacterial

   Promoter

   pPhlF

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-ERK2 K52R

   Plasmid

   #118298

   Purpose

   Expression of Rat ERK2

   Depositor

   Philip Stork

   Article

   Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

   Insert

   ERK2 K52R (Mapk1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K52R

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ839

   Plasmid

   #39317

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (NMA0631 Neisseria meningitidis)
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

5. p2T-CMV-BE4max-BlastR

   Plasmid

   #152991

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. IBMc460

   Plasmid

   #161940

   Purpose

   Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
   

   Expression

   Bacterial

   Promoter

   PPhlF

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-104

   Plasmid

   #79842

   Purpose

   Expresses NLS-PpsR2-VP16 under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16
   

   Tags

   Nuclear localization signal of nuclear cap-bindin…

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV/EF1a-DIO-SCLM

   Plasmid

   #216759

   Purpose

   AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

   Depositor

   George Augustine

   Insert

   SuperClomeleon
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   EF1a (human elongation factor 1 alpha)

   Available Since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

   Plasmid

   #178868

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-PB1-AG(Y63L)-LOV2

    Plasmid

    #178876

    Purpose

    Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen(Y63L)-LOV2
    

    Expression

    Mammalian

    Mutation

    AzamiGreen: Y63L

    Promoter

    CAG

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-FRISZ-TM

    Plasmid

    #176886

    Purpose

    AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    Ig kappa-FRISZ-PDGFR TM
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 FLIPE-100u

    Plasmid

    #13543

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCL.028

    Plasmid

    #184965

    Purpose

    Test effect of extending -Eco1 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRG710_lexO-HO_LexA-TF_HIS3MX

    Plasmid

    #154819

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ-NF-L1-dMb2Cas12a

    Plasmid

    #177683

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible foki-dmb2cas12a entry …

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. px459 sgFIP200

    Plasmid

    #175024

    Purpose

    CRISPR-Cas9 plasmid targeting exon 3 of human FIP200.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    RB1CC1 (RB1CC1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a_Pro-IL-36α

    Plasmid

    #214343

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36A (IL36A Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7-RelA(K221R)

    Plasmid

    #23248

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    NFkB RelA (RELA Human)
    

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    K221R

    Available Since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pJG380

    Plasmid

    #91188

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9+gNt_R2+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTP443

    Plasmid

    #216361

    Purpose

    xNup98-Nb1t (+1Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xNup98-Nb1t (+1Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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