We narrowed to 16,144 results for: LEA
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WT (REP10)
Plasmid#21368DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
ExpressionMammalianAvailable SinceJan. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-42
Plasmid#172758PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-42
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ-mNeongreen
Plasmid#182392PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusionDepositorInsertVNp-LZ-mNeongreen
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1A-BRD3R
Plasmid#130696PurposeLentiviral plasmids encoding human BRD3RDepositorInsertHuman BRD3R (BRD3 Human)
UseLentiviralAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-E795L
Plasmid#176890PurposeGateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoterDepositorInsertLbCas12a-E795L
UseCRISPR; Gateway compatible lbcpf1 entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-6
Plasmid#172741PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-6
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
mRFP-LAP2β
Plasmid#228029Purposelentiviral construct for mRFP-LAP2β expressionDepositorAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_deltaOB
Plasmid#166408Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DeltaN24
Plasmid#214327PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
TagsHis-TEVExpressionBacterialMutationDeletion of first 24 amino acidsPromoterT7Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_human-pro-IL-18
Plasmid#214337PurposeProtein overexpression in bacteriaDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAGFP
Plasmid#58255PurposeU6 promoter-driven single guide RNA complementary to eGFPDepositorInsertU6.gRNAGFP cassette
UseAdenoviral and CRISPRExpressionMammalianAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV U6-sgRNA UbC-tdTomato
Plasmid#106949PurposesgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato markerDepositorInsertsgRNA BsmBI/Esp3I cloning site
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-LOx-IRES-EGFP
Plasmid#166877PurposeExpress the bacterial lactate oxidase (LOx) enzyme in mammalian cellsDepositorInsertLactate oxidase
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin
Plasmid#134863PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-rnc-sfGFP
Plasmid#51560PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).DepositorInsertrnc (rnc E. coli)
TagsGLESTCRHASLAVLADERRFSA and superfolder GFPExpressionBacterialMutationContains additonal aa at the end of the sfGFP in …Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only
