We narrowed to 17,420 results for: SHE

1. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

   Plasmid

   #178048

   Purpose

   CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Lrig1 shRNAmir
   

   Use

   Cre/Lox and RNAi; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

   Plasmid

   #178047

   Purpose

   CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Lrig1 shRNAmir
   

   Use

   Cre/Lox and RNAi; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNHA_OROV_NCP

   Plasmid

   #208937

   Purpose

   Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells

   Depositor

   Gerald Barry

   Insert

   Nucleocapsid
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted start codon so HA included at N-terminus

   Promoter

   CMV

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-hMYF5 (#795)

   Plasmid

   #78333

   Purpose

   Overexpression of human MYF5 ORF with a N-terminal FLAG tag

   Depositor

   Louis Kunkel, Matthew Alexander

   Insert

   MYF5 (MYF5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

   Plasmid

   #217305

   Purpose

   sgRNA BFP + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-BFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   U6, Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-mNeonGreen

   Plasmid

   #124101

   Purpose

   Expression of the fluorescent protein mNeonGreen in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Insert

   mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Codon usage was optimized for Homo Sapiens

   Promoter

   CAG

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPN1‐mTim1

   Plasmid

   #49215

   Purpose

   expresses EGFP tagged mouse Tim1

   Depositor

   Lawrence Kane

   Insert

   Tim1 (Havcr1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PTPN5

   Plasmid

   #39169

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PTPN5 (PTPN5 Human)
   

   Use

   Tags

   GST-PreScission

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pACP-tag (m)- 2 Vector

   Plasmid

   #101126

   Purpose

   Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of ACP-tag.

   Depositor

   New England Biolabs, Ana Egana

   Type

   Empty backbone

   Use

   Tags

   ACP-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. p72B9-FBL-HC

    Plasmid

    #194637

    Purpose

    Expresses 72B9 hybridoma anti-FBL antibody, heavy chain

    Depositor

    Susan Baserga

    Insert

    72B9 antibody, heavy chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p72B9-FBL-LC

    Plasmid

    #194638

    Purpose

    Expresses 72B9 hybridoma anti-FBL antibody, light chain

    Depositor

    Susan Baserga

    Insert

    72B9 antibody, light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJR255

    Plasmid

    #78549

    Purpose

    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.

    Depositor

    John Rossi

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CMV

    Available since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mT2A-tdTomato-FNF

    Plasmid

    #182340

    Purpose

    Recombineering cassette plasmid for T2A-tdTomato-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-tdTomato-FNF
    

    Use

    Mouse Targeting; Flp / frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22B_PcrA_2Bchimera

    Plasmid

    #102998

    Purpose

    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

    Depositor

    Martin Webb

    Insert

    PcrA_2Bchimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Bacilllus stearothermophilus PcrA in which the 2B…

    Promoter

    T7

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCP-tag (m) Vector

    Plasmid

    #101127

    Purpose

    Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of MCP-tag.

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Use

    Tags

    MCP-tag (Mutant ACP- tag)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pHD-w+

    Plasmid

    #80927

    Purpose

    Vector for generating dsDNA donors for HDR. Empty backbone containing a StuI restriction site and GMR-white for identifying backbone incorporation.

    Depositor

    Kate O'Connor-Giles

    Insert

    GMR-white
    

    Use

    High copy, amp resistance

    Tags

    Expression

    Mutation

    Promoter

    GMR::hsp70

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3_SARS2_EG.5.1

    Plasmid

    #207641

    Purpose

    pseudovirus plasmid that expresses spike of EG.5.1

    Depositor

    David Nemazee

    Insert

    Spike (EG.5.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q52H, F456L on the basis of XBB.1.5

    Promoter

    CMV

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEos3.2-Rad21-N

    Plasmid

    #66951

    Purpose

    Localization: Centromere, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    Rad21 (RAD21 Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pBEST-PLtetO-1-UTR1-TetR-deGFP-T500

    Plasmid

    #45774

    Depositor

    Richard Murray

    Insert

    tetR-deGFP
    

    Use

    Synthetic Biology

    Tags

    Fusion protein of TetR and deGFP, with glycine-se…

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9CR4-NG

    Plasmid

    #120225

    Purpose

    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

    Depositor

    Christopher Reisch

    Insert

    SpCas9-NG
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

    Promoter

    pTet

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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