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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 164,459 results for: Des

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Showing: 5101 - 5120 of 164459 results

  4. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  7. SG3ΔENV CA E28A,E29A

    Plasmid
    #145785
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E28A,E29A mutation in capsid (CA E28A,E29A).
    Depositor
    Wesley Sundquist
    Article
    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/
    Insert
    Capsid
    Use
    Lentiviral and Retroviral
    Expression
    Mammalian
    Mutation
    Changed Capsid residues Glutamic Acid residues 28…
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    145785-plasmid-map-sequence-id-313273

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  12. pMSP1D1R1

    Plasmid
    #97323
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1R1
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of R residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97323-plasmid-map-sequence-id-182662

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  15. mCit-APPAP-gs

    Plasmid
    #228090
    Purpose
    Mammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAP
    Expression
    Mammalian
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228090-plasmid-map-sequence-id-451134

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  17. pMSP1D1G1

    Plasmid
    #97325
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1G1
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of G residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97325-plasmid-map-sequence-id-182623

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Showing: 5101 - 5120 of 164459 results