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pAAV-S5E2-GFP-fGFP (AAV9)

Viral prep

#135631-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Tags

EGFP

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only
pCTCON2-BXG-2TAG

Plasmid

#158138

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMy-BirA-T2A-eGFP

Plasmid

#50565

Purpose

Retroviral vector encoding for GFP and the biotinylating enzyme BirA

Depositor

Rafael Casellas

Publication

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pYS2

Plasmid

#117490

Purpose

Generation of unmarked gene deletions in Mycobacterium smegmatis

Depositor

Eyal Gur

Publication

Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

Insert

gfp, hyg

Use

Deletion generation

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEO100c

Plasmid

#50550

Purpose

Expresses sfGFP under the PompC1157 promoter

Depositor

Jeffrey Tabor

Publication

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Tet-pLKO.1-puro-shGFP

Plasmid

#247239

Purpose

shRNA control targeting GFP

Depositor

Miguel Ramalho-Santos

Publication

Cho et al Oncogene. 2026 Apr;45(13):1198-1210. doi

Insert

negtive control shRNA targeting GFP

Use

Lentiviral

Expression

Mammalian

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pHBlaM_GFP

Plasmid

#223809

Purpose

inducible expression of high-copy BlaM (cytoplasmic variant) and constitutive expression of GFP

Depositor

Lingchong You

Publication

Ma et al Nat Commun. 2024 Sep 27;15(1):8337. doi:

Inserts

superfolder GFP
BlaM

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBlaM_GFP

Plasmid

#223807

Purpose

inducible expression of low-copy BlaM (cytoplasmic variant) and constitutive expression of GFP

Depositor

Lingchong You

Publication

Ma et al Nat Commun. 2024 Sep 27;15(1):8337. doi:

Inserts

superfolder GFP
BlaM

Use

Unspecified

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

Plasmid

#129668

Purpose

A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Publication

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

GFP

Expression

Mammalian

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CMV-GFP-CaM-uTEVp

Plasmid

#163028

Purpose

FLiCRE protease for HEK293T expression

Depositor

Alice Ting

Publication

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-uTEVp

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-PASS

Plasmid

#193970

Purpose

Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Publication

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LI D-E GFP + linker c3

Plasmid

#54410

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + GFP

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBbB3a-GFP

Plasmid

#35346

Depositor

Jay Keasling

Publication

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProS

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-fTCAP-GFP

Plasmid

#22916

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu titan cap promoter driving GFP

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pPSt_35Spr-GFP-t35S

Plasmid

#203592

Purpose

Fully assembled Plant STARR-seq plasmid with the 35S terminator.

Depositor

Stan Fields

Publication

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Inserts

CaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator

Expression

Plant

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP-TAG

Plasmid

#158131

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Mutation

Introduced a stop codon in place of the tyrosine …

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-CAG-GFP-EnvA

Plasmid

#102987

Purpose

Lentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies

Depositor

Marco Tripodi

Publication

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

Enhanced Green Fluorescent Protein (eGFP)
EnvA-B19 Rabies Glycoprotein

Use

Lentiviral

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDAS12395_PEAR-GFP_2.0-preedited

Plasmid

#177185

Purpose

PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Publication

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSL1180-NiV L(2k)-mCherry/eGFP

Plasmid

#234654

Purpose

Minigenome plasmid of NiV Bangladesh strain

Depositor

Carolina Lopez

Publication

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Inserts

part L gene
mCherry
eGFP

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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