We narrowed to 15,824 results for: LEA

1. pOCC167

   Plasmid

   #118909

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1p shDna2

   Plasmid

   #31951

   Depositor

   Sheila Stewart

   Article

   Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

   Insert

   shRNA against hDna2 (DNA2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

3. p2xFlag CMV2-YAP2-deltaC

   Plasmid

   #21123

   Depositor

   Marius Sudol

   Article

   Tsutomu Oka & Marius et al

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   5 Carboxyterminal amino acids -FLTWL are deleted

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pRSET-TEV-12His

   Plasmid

   #137032

   Purpose

   empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Type

   Empty backbone

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CMV-CIBN-mCherry-VAMP2

   Plasmid

   #178573

   Purpose

   Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CIBN-mCherry-VAMP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKC-delta-IRES-hrGFP

   Plasmid

   #73414

   Purpose

   Mammalian expression of mouse PKC-delta and hrGFP

   Depositor

   Raymond Swanson

   Article

   Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

   Insert

   PKC-delta (Prkcd Mouse)
   

   Use

   Tags

   3xFLAG and IRES-hrGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pY006 (pcDNA3.1-hBpCpf1)

   Plasmid

   #69978

   Purpose

   Expresses humanized BpCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hBpCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-JNKAR1-NES

   Plasmid

   #84632

   Purpose

   Unimolecular, FRET-based JNK activity reporter; cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ECFP-FHA1-JNKsubDD-Citrine-NES
   

   Use

   Tags

   6xHis, Citrine, ECFP, Nuclear export signal (NES)…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABEpuro-Flag-ZO-2

   Plasmid

   #27420

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

10. 3535 pET15b Bid p22

    Plasmid

    #8784

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p22 (Bid Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pY013 (pcDNA3.1-hEeCpf1)

    Plasmid

    #69985

    Purpose

    Expresses humanized EeCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hEeCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pNIC-Bio2_SMAC(delta(55))_9His

    Plasmid

    #197976

    Purpose

    E. coli expression of SMAC(delta(55))-9His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    Smac (DIABLO Human)
    

    Use

    Tags

    9His

    Expression

    Bacterial

    Mutation

    delta (1-55)

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY019 (pcDNA3.1-hPmCpf1)

    Plasmid

    #69991

    Purpose

    Expresses humanized PmCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPmCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pY009 (pcDNA3.1-hSsCpf1)

    Plasmid

    #69981

    Purpose

    Expresses humanized SsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hSsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pAYC

    Plasmid

    #62657

    Purpose

    Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. cycLuc_SuMMVs

    Plasmid

    #119210

    Purpose

    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with SuMMVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

    Plasmid

    #129704

    Purpose

    Express HA-KASH for nuclei labeling and purification

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    HA-VHH-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. R26-EGFP MMEJ donor vector

    Plasmid

    #137926

    Purpose

    R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with micro-homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLaNGo11-1

    Plasmid

    #126053

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3495 pcDNA3 Bid p15

    Plasmid

    #8780

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only