We narrowed to 15,981 results for: LEA

1. pDY-HA1-HA2-neo

   Plasmid

   #109123

   Purpose

   FR cassette provider to introduce FR cassette to a BAC by recombineering

   Depositor

   David Gilbert

   Article

   Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

   Inserts

   HA1
   HA2
   

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry_LD 0_CS G_tTA-BFP

   Plasmid

   #58856

   Purpose

   MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY007 (pcDNA3.1-hPeCpf1)

   Plasmid

   #69979

   Purpose

   Expresses humanized PeCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hPeCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1V5-His Snk/Plk2 D223N

   Plasmid

   #22218

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, aspartic a…

   Available Since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pY008 (pcDNA3.1-hPbCpf1)

   Plasmid

   #69980

   Purpose

   Expresses humanized LPbCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hPbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pMKO.1-shMki67

   Plasmid

   #160958

   Purpose

   Mki67 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Mki67 (Mki67 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mouse Cavin-1

   Plasmid

   #50441

   Purpose

   expresses mouse Cavin-1 in mammalian cells

   Depositor

   Paul Pilch

   Article

   Liu et al J Biol Chem. 2008 Feb 15;283(7):4314-22.

   Insert

   Cavin-1 (Cavin1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP-C1X-Nup93-mut

   Plasmid

   #87335

   Purpose

   To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP93 (NUP93 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. IBMc071

    Plasmid

    #161943

    Purpose

    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIPZ-DoxOn-shCUX1-5150

    Plasmid

    #90469

    Purpose

    Lentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099

    Depositor

    Alain Nepveu

    Article

    Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

    Insert

    TGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV PIG IMP-1-long-mut

    Plasmid

    #21661

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    mutated let-7 binding sites.

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL3.7 shRNA against CRTC3

    Plasmid

    #72712

    Purpose

    Lentiviral expression of shRNA against CRTC3 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC3
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3HA-AMOT p130∆PPxY1

    Plasmid

    #166711

    Purpose

    expresses AMOT with inactivating mutation in first PPxY motif in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 L129A P130A
    

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed leucine 129 to alanine, proline 130 to al…

    Promoter

    CMV promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-24

    Plasmid

    #172750

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-24
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-9

    Plasmid

    #172742

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-9
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30a-SRAP

    Plasmid

    #84524

    Purpose

    Expresses murine SRAP domain (250 aa) in bacteria

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    SRAP domain (Hmces Mouse)
    

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBCV

    Plasmid

    #62659

    Purpose

    Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    FLPo
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. cycLuc_SbMVs

    Plasmid

    #119209

    Purpose

    Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with SbMVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET41a iGluf

    Plasmid

    #119833

    Purpose

    Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluf
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25D

    Promoter

    T7

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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