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pcDNA3-Flag-RNF168 delta MIU1/MIU2

Plasmid

#133981

Purpose

mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1 and MIU2

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-RNF168 delta MIU2

Plasmid

#133979

Purpose

mammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU2

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLV 4xSTAR-mTagBFP2-blast

Plasmid

#125239

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTagBFP2 (codon optimized)

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGL403

Plasmid

#119953

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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12xMoonTag-12xSunTag-kif18b-24xPP7

Plasmid

#128606

Purpose

Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

12xMoonTag-12xSunTag
kinesin family member 18b (KIF18B Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMIG-IRF4

Plasmid

#58987

Purpose

MSCV based expression vector - co-expresses GFP and murine IRF4

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Insert

Mouse IRF4 (Irf4 Mouse)

Use

Retroviral

Tags

Kozak sequence added

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-mCerulean

Plasmid

#154951

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

Plasmid

#154773

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Mutation

Y125A in G1PylS, hybrid PylT with A41AA and C55A …

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_1

Plasmid

#195130

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 1 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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GLI1_pLENTI-CAG-IRES-GFP

Plasmid

#176992

Purpose

Mammalian lentiviral expression vector encoding GLI1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-PSMD4

Plasmid

#202666

Purpose

Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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DOP1B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176979

Purpose

Mammalian expression vector encoding DOP1B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DOP1B (DOPEY2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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IFNAR2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176938

Purpose

Mammalian expression vector encoding IFNAR2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

IFNAR2 (IFNAR2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

Plasmid

#47904

Purpose

An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

eNpHR3.0

Use

AAV

Tags

iRFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_2

Plasmid

#195131

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 2 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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CaMKII-somBiPOLES-mCerulean

Plasmid

#154948

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

CaMKIIa(0.4)

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flt3-ITD-Y591F/Y919F

Plasmid

#74499

Purpose

Retroviral expression of FLT3 ITD-Y591F/Y919F

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

unknown

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

Plasmid

#170195

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Promoter

CMV51

Available Since

Nov. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Tags

Flag

Expression

Mammalian

Promoter

pCMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pNon-target

Plasmid

#214053

Purpose

Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Non-target sequence

Use

Non-target rna plasmid

Expression

Bacterial

Promoter

trc promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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